ID   T2G1_AVIVO              Reviewed;         488 AA.
AC   P43418;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Type II restriction enzyme HgaI {ECO:0000303|Ref.1};
DE            Short=R.HgaI {ECO:0000303|Ref.1};
DE            EC=3.1.21.4 {ECO:0000305|Ref.1};
DE   AltName: Full=Endonuclease HgaI {ECO:0000303|Ref.1};
DE   AltName: Full=Type-2 restriction enzyme HgaI;
GN   Name=hgaIR;
OS   Avibacterium volantium (Pasteurella volantium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Avibacterium.
OX   NCBI_TaxID=762;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 14385 / DSM 22841 / CCUG 3713 / NCTC 3438 / Lovell 6;
RA   Sugisaki H.;
RT   "Nucleotide sequence of the gene of HgaI restriction endonuclease.";
RL   Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: An S subtype restriction enzyme that recognizes the double-
CC       stranded sequences 5'-GACGC-3' and 5'-GCGTC-3' and cleaves respectively
CC       10 bases after G-1 and 10 bases before G'-1. {ECO:0000269|Ref.1,
CC       ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000305|Ref.1};
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DR   EMBL; D17388; BAA04208.1; -; Genomic_DNA.
DR   PIR; C59240; C59240.
DR   AlphaFoldDB; P43418; -.
DR   PRO; PR:P43418; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..488
FT                   /note="Type II restriction enzyme HgaI"
FT                   /id="PRO_0000077309"
SQ   SEQUENCE   488 AA;  56737 MW;  B5E05259E3C710D2 CRC64;
     MEKILMLNDD QIWIFKKHTN NIQLLIEVAL YLKSNKSSVS KKDKDAMYDI FSESELYNPR
     ESLRDKPLDT INHKLDGLSY FMFGYSDRIN DENKFIFSPL GNLFLKYLHD KDKLSKIFSC
     MLISMQFPHP YSKPSECFLL YPFRLIFKLL LDKRLQGRLY HYEVYKIIIH TISIDEAKYE
     FLVKSILNSR KKSWNEKLNE LSEIQHKVVK SVYEWQYYIV PLLGSLHIFK INNGDIEQKL
     YHPQKDGSKS PPTARKANNG YVEINDNLTN FIDKLLNKYS FLDTPILLSD SQRKSNDVTK
     EIYSFYPELL LAEIGETISF ESHILNIPKL ITEYSKNPDN STSGKFEKIL EEAFNLFIDV
     EAQWLAGAGR TDIECMYLPI NEKFSIEAKS TKNKLSMINS GRLKRHRTLI SANYTIVITP
     RYVPSVRYDI EAQDIVLITA DTLAEYLYNN IISNNRDISY ADIQAIIVAN LGKDISTQIS
     NLTLSKFG