T2H1_HAEPA
ID T2H1_HAEPA Reviewed; 254 AA.
AC P29537;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=Type II restriction enzyme HpaI {ECO:0000303|PubMed:12654995};
DE Short=R.HpaI {ECO:0000303|PubMed:1542567};
DE EC=3.1.21.4;
DE AltName: Full=Endonuclease HpaI;
DE AltName: Full=Type-2 restriction enzyme HpaI;
GN Name=hpaIR;
OS Haemophilus parainfluenzae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=729;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22, AND FUNCTION.
RC STRAIN=ATCC 49669;
RX PubMed=1542567; DOI=10.1093/nar/20.4.705;
RA Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.;
RT "Cloning and expression of the HpaI restriction-modification genes.";
RL Nucleic Acids Res. 20:705-709(1992).
RN [2]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded sequence 5'-GTTAAC-3' and cleaves after T-3.
CC {ECO:0000269|PubMed:1542567, ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR EMBL; D10668; BAA01518.1; -; Genomic_DNA.
DR PIR; S28680; S28680.
DR AlphaFoldDB; P29537; -.
DR REBASE; 1158; HpaI.
DR PRO; PR:P29537; -.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW Restriction system.
FT CHAIN 1..254
FT /note="Type II restriction enzyme HpaI"
FT /id="PRO_0000077324"
SQ SEQUENCE 254 AA; 29629 MW; C196D22A771B9CED CRC64;
MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK KGLPLENINK
IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD IILQFQVNNN VITGNVDVKA
TSNDIPDSGK SPNITSFSRI RTAYVKDPNF IFIILSIKHS VYVKRNEYTN LMDGIMQIID
FNVYDLKYIS DSDISYNPAL GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE
QFYNEAKRNK WIKD