ID   T2H2_HAEAE              Reviewed;         352 AA.
AC   O30869;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Type II restriction enzyme HaeII {ECO:0000303|PubMed:12654995};
DE            Short=R.HaeII;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease HaeII;
DE   AltName: Full=Type-2 restriction enzyme HaeII;
GN   Name=haeIIR; Synonyms=dcrA;
OS   Haemophilus aegyptius.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=197575;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11116 / CCUG 25716 / NCTC 8502 / 180-a;
RX   PubMed=9628358;
RA   Stein D.C., Gunn J.S., Piekarowicz A.;
RT   "Sequence similarities between the genes encoding the S.NgoI and HaeII
RT   restriction/modification systems.";
RL   Biol. Chem. 379:575-578(1998).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-RGCGCY-3' and cleaves after C-5.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; AF019752; AAB70830.1; -; Genomic_DNA.
DR   RefSeq; WP_065250747.1; NZ_UGHG01000002.1.
DR   AlphaFoldDB; O30869; -.
DR   REBASE; 1088; HaeII.
DR   PRO; PR:O30869; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019058; Restrct_endonuc_II_HaeII.
DR   Pfam; PF09554; RE_HaeII; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..352
FT                   /note="Type II restriction enzyme HaeII"
FT                   /id="PRO_0000077316"
SQ   SEQUENCE   352 AA;  40801 MW;  3AE01F59FB1C289A CRC64;
     MNDIQVAKES LDKIIKKARV HLYKPIQIAE ILYQDRVNKN INLLDKETYR NISKKWRDVI
     CIRFLGRVST SSAKYQDDLF NDNAMPPKHL NILGEMNRKT NGGVESYIYK KFFERFNQMS
     SALEYSYSRT PDNFHLSEFL ALFWLEPGLK RSIDKVYEIV VYALFSSLIE ALGVKVKIDL
     DLSNIDLLKE FEDFTRQIIS LDSENTSLEL NAKINRVGVT NASDRGLDMW ANFGMAIQIK
     HLSLTEELAE NIVSSVSSDR IVIVCKESEE KLILSLLNQI GWRSKIQSII TEADLIKWYD
     KALRGKSAYL VGSKILEHIR NEINLEFPAT DIVDFNHFFH EREYNKALDI EK