ID   T2H3_HAEAE              Reviewed;         318 AA.
AC   O68584;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Type II restriction enzyme HaeIII {ECO:0000303|PubMed:12654995};
DE            Short=R.HaeIII;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease HaeIII;
DE   AltName: Full=Type-2 restriction enzyme HaeIII;
GN   Name=haeIIIR;
OS   Haemophilus aegyptius.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=197575;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11116 / CCUG 25716 / NCTC 8502 / 180-a;
RA   Zhang B.-H., Wilson G.G.;
RL   Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGCC-3' and cleaves after G-2.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; AF051375; AAC05697.1; -; Genomic_DNA.
DR   RefSeq; WP_006996034.1; NZ_UGHG01000002.1.
DR   AlphaFoldDB; O68584; -.
DR   SMR; O68584; -.
DR   REBASE; 1089; HaeIII.
DR   PRO; PR:O68584; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019059; Restrct_endonuc_II_HaeIII.
DR   Pfam; PF09556; RE_HaeIII; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..318
FT                   /note="Type II restriction enzyme HaeIII"
FT                   /id="PRO_0000077317"
SQ   SEQUENCE   318 AA;  37129 MW;  10E35B4B51F977DD CRC64;
     MSNKSNDNGR AYEFAFINEL GRIATQNQNI NIEKNSSYYV VEKSWSTLSD LEKEKYTKSA
     IAGINLITSL EPIIEDGNGV LNLKIQADNK GELGDIRDIL IQRENINWEI GLSLKHNHFA
     VKHSRLSHKI DFSEKWFQLP SSQNYWDNIL PIFEKLEIYK KDKIKWRELS NKEDCIYYPI
     LKSFIAEIKE KYDKYNSIVP QRMVEYLLGY FDFYKIISQD NKKLTSIQSF NLRGTLNKPS
     KKRKADIFIP VANLPTRIID IDFKPNSKNT VELYLDKGWQ FSFRIHNAST IIEPSLKFDI
     KLIGVPATII CLETPWEE