ID   T2L2_LACLC              Reviewed;         304 AA.
AC   P50177;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Type II restriction enzyme LlaDCHI {ECO:0000303|PubMed:12654995};
DE            Short=R.LlaDCHI;
DE            EC=3.1.21.4 {ECO:0000305|PubMed:7793939};
DE   AltName: Full=Endonuclease LlaDCHI;
DE   AltName: Full=LlaII;
DE   AltName: Full=Type-2 restriction enzyme LlaDCHI;
GN   Name=llaDCHIR; Synonyms=llaIIC {ECO:0000303|PubMed:7793939};
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OG   Plasmid pSRQ700.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=DCH-4;
RX   PubMed=7793939; DOI=10.1128/aem.61.6.2193-2202.1995;
RA   Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.;
RT   "Cloning and sequencing of LlaDCHI restriction/modification genes from
RT   Lactococcus lactis and relatedness of this system to the Streptococcus
RT   pneumoniae DpnII system.";
RL   Appl. Environ. Microbiol. 61:2193-2202(1995).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded unmethylated sequence 5'-GATC-3' and cleaves before G-1.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:7793939}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000305|PubMed:7793939};
CC   -!- MISCELLANEOUS: The LlaDCHI restriction system has two different
CC       methylases. {ECO:0000269|PubMed:7793939}.
CC   -!- MISCELLANEOUS: Genes encoded on plasmid pSQR700 confer strong
CC       resistance to the three most common lactococcal phage species (936, c2,
CC       and P335). Its presence is probably one reason for the strong
CC       bacteriophage resistance shown by strain DCH-4 over the years.
CC       {ECO:0000305|PubMed:7793939}.
CC   -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; U16027; AAB06313.1; -; Genomic_DNA.
DR   RefSeq; NP_116731.1; NC_002798.1.
DR   AlphaFoldDB; P50177; -.
DR   REBASE; 249491; WciM2ORF523P.
DR   REBASE; 2772; LlaDCHI.
DR   PRO; PR:P50177; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR   InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR   Pfam; PF04556; DpnII; 1.
DR   PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Restriction system.
FT   CHAIN           1..304
FT                   /note="Type II restriction enzyme LlaDCHI"
FT                   /id="PRO_0000077328"
SQ   SEQUENCE   304 AA;  34670 MW;  C0C84547A28A20E4 CRC64;
     MDFNNYIGLE SDDRLNAFMA TLSVTNRTPE YYVNWEKVER ETRKFELELN TLNYLIGKED
     IYSEALELFT NQPELLKAIP SLIASRDTSL DILNIDENDD MSFEQLNFLV IDENCIADYV
     DFINQAGLLD FLQNKAKRSL VDYVYGVEAG LDSNARKTRS GTTMEGILER TVSKIAQGKG
     LDWKPQATAS FIKSQWDIEV PVDKSKRRFD AAVYSRALNK VWLIETNYYG GGGSKLKAVA
     GEFTELSQFV KTSKDNVEFV WVTDGQGWKF SRLPLAEAFG HIDNVFNLTM LKEGFLSDLF
     EKEI