ID   T2M1_METJA              Reviewed;         222 AA.
AC   Q58391;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Type II restriction enzyme MjaI {ECO:0000303|PubMed:12654995};
DE            Short=R.MjaI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease MjaI;
DE   AltName: Full=Type-2 restriction enzyme MjaI;
GN   Name=mjaIR; OrderedLocusNames=MJ0984;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   CHARACTERIZATION.
RA   Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D.;
RT   "Method for screening restriction endonucleases.";
RL   Patent number WO9911821, 11-MAR-1999.
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CTAG-3'; the cleavage site is unknown.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; L77117; AAB98987.1; -; Genomic_DNA.
DR   PIR; H64422; H64422.
DR   AlphaFoldDB; Q58391; -.
DR   REBASE; 1221; MjaI.
DR   EnsemblBacteria; AAB98987; AAB98987; MJ_0984.
DR   KEGG; mja:MJ_0984; -.
DR   eggNOG; arCOG11279; Archaea.
DR   HOGENOM; CLU_120987_0_0_2; -.
DR   InParanoid; Q58391; -.
DR   OMA; WEEYYET; -.
DR   PRO; PR:Q58391; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019068; Restrct_endonuc_II_MjaI.
DR   Pfam; PF09568; RE_MjaI; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Restriction system.
FT   CHAIN           1..222
FT                   /note="Type II restriction enzyme MjaI"
FT                   /id="PRO_0000077332"
SQ   SEQUENCE   222 AA;  25962 MW;  9A254A208AB8AC02 CRC64;
     MVKLMKKLED KKGIIEITFE EEREILELPS KPELPKYASQ LINLANIFSQ GTRPKVVGQM
     SELIKEFRKT GGRTFEDWKK WYLQKYPNAI DEATEKIWNM LNNFKETLEQ LERDDVRKWV
     EDLVLIKTYE GLMLQDAILK KVAEELGGNY RPSTIEEESK GIDGVIIIDD KEIPVSIKSK
     TYVNQEKHLS EELKGHLIIY EKKKNKIIVD YSDLLDLVEN TK