ID   T2M1_MICAM              Reviewed;         308 AA.
AC   P50189;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Type II restriction enzyme MamI {ECO:0000303|PubMed:12654995};
DE            Short=R.MamI {ECO:0000303|PubMed:8654988};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease MamI;
DE   AltName: Full=Type-2 restriction enzyme MamI;
GN   Name=mamIR {ECO:0000303|PubMed:8654988};
OS   Microbacterium ammoniaphilum.
OC   Bacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium.
OX   NCBI_TaxID=42460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-24.
RC   STRAIN=ATCC 15354 / DSM 20156 / BCRC 11670 / NCIMB 10335 / NRRL B-4247;
RX   PubMed=8654988; DOI=10.1016/0378-1119(96)00189-8;
RA   Striebel H.-M., Seeber S., Jarsch M., Kessler C.;
RT   "Cloning and characterization of the MamI restriction-modification system
RT   from Microbacterium ammoniaphilum in Escherichia coli.";
RL   Gene 172:41-46(1996).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GATNNNNATC-3' and cleaves after N-5.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; X79027; CAA55648.1; -; Genomic_DNA.
DR   PIR; T45133; T45133.
DR   AlphaFoldDB; P50189; -.
DR   REBASE; 1200; MamI.
DR   PRO; PR:P50189; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019067; Restrct_endonuc_II_MamI.
DR   Pfam; PF09567; RE_MamI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   CHAIN           1..308
FT                   /note="Type II restriction enzyme MamI"
FT                   /id="PRO_0000077329"
SQ   SEQUENCE   308 AA;  34293 MW;  53057207FBF1BB92 CRC64;
     MQNAVSQAIS QGIHVRREIL GSLTYEQRVF LLEDLFVDLF GHQHVMLQRW AALTGQSAQV
     DTGYIAQFVA SIVLGEPGQG FRGKGDDLAD GSEVKSAANI SGVDRPRWNH NLGSLDDDEH
     RRSRGLPTAG EEYLGVPYMF YLLVDRPHGV SDPAPIRIRA WCIDAQEDGD WRDLFETFLT
     SRRGRTYNFQ LHPPVGYDDD VVVNTLGNLD FSNVLVFDAR LSLADRDRPE IDWHVPLPTQ
     VIPVTGRTRA LRYGGRGARP TRLTNTADIV LGTNDLGALF PGVLAPRDSY DLATVSEIET
     EAEVEEYS