T2M1_MORSP
ID T2M1_MORSP Reviewed; 262 AA.
AC P11405;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Type II restriction enzyme MspI {ECO:0000303|PubMed:12654995};
DE Short=R.MspI;
DE EC=3.1.21.4 {ECO:0000305|PubMed:2471145};
DE AltName: Full=Endonuclease MspI;
DE AltName: Full=Type-2 restriction enzyme MspI;
GN Name=mspIR;
OS Moraxella sp.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Moraxella; unclassified Moraxella.
OX NCBI_TaxID=479;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 49670;
RX PubMed=2471145; DOI=10.1093/nar/17.8.3001;
RA Lin P.M., Lee C.H., Roberts R.J.;
RT "Cloning and characterization of the genes encoding the MspI restriction
RT modification system.";
RL Nucleic Acids Res. 17:3001-3011(1989).
RN [2]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded sequence 5'-CCGG-3' and cleaves after C-1.
CC {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:2471145}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC Evidence={ECO:0000305|PubMed:2471145};
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DR EMBL; X14191; CAA32394.1; -; Genomic_DNA.
DR PIR; S04187; NDKE2M.
DR PDB; 1SA3; X-ray; 1.95 A; A/B=1-262.
DR PDB; 1YFI; X-ray; 2.70 A; A/B=1-262.
DR PDBsum; 1SA3; -.
DR PDBsum; 1YFI; -.
DR AlphaFoldDB; P11405; -.
DR SMR; P11405; -.
DR REBASE; 1277; MspI.
DR EvolutionaryTrace; P11405; -.
DR PRO; PR:P11405; -.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR015291; Restrct_endonuc_II_MspI.
DR Pfam; PF09208; Endonuc-MspI; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endonuclease; Hydrolase; Nuclease; Restriction system.
FT CHAIN 1..262
FT /note="Type II restriction enzyme MspI"
FT /id="PRO_0000077338"
FT HELIX 4..12
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 23..41
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 45..51
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 57..69
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 77..84
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 100..106
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 111..124
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 126..131
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 133..140
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 146..157
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 165..175
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 179..188
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 202..210
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 213..221
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 222..234
FT /evidence="ECO:0007829|PDB:1SA3"
FT HELIX 239..242
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 244..249
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 252..255
FT /evidence="ECO:0007829|PDB:1SA3"
FT STRAND 258..262
FT /evidence="ECO:0007829|PDB:1SA3"
SQ SEQUENCE 262 AA; 29829 MW; E724275DCE50E6E8 CRC64;
MRTELLSKLY DDFGIDQLPH TQHGVTSDRL GKLYEKYILD IFKDIESLKK YNTNAFPQEK
DISSKLLKAL NLDLDNIIDV SSSDTDLGRT IAGGSPKTDA TIRFTFHNQS SRLVPLNIKH
SSKKKVSIAE YDVETICTGV GISDGELKEL IRKHQNDQSA KLFTPVQKQR LTELLEPYRE
RFIRWCVTLR AEKSEGNILH PDLLIRFQVI DREYVDVTIK NIDDYVSDRI AEGSKARKPG
FGTGLNWTYA SGSKAKKMQF KG
