ID   BPHA2_PSES1             Reviewed;         193 AA.
AC   Q52439;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Biphenyl dioxygenase subunit beta;
DE            EC=1.14.12.18;
DE   AltName: Full=Biphenyl 2,3-dioxygenase;
GN   Name=bphA2;
OS   Pseudomonas sp. (strain KKS102).
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=307;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8048958; DOI=10.1006/bbrc.1994.2008;
RA   Fukuda M., Yasukochi Y., Kikuchi Y., Nagata Y., Kimbara K., Horiuchi H.,
RA   Takagi M., Yano K.;
RT   "Identification of the bphA and bphB genes of Pseudomonas sp. strains
RT   KKS102 involved in degradation of biphenyl and polychlorinated biphenyls.";
RL   Biochem. Biophys. Res. Commun. 202:850-856(1994).
CC   -!- FUNCTION: The beta subunit may be responsible for the substrate
CC       specificity of the enzyme.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=biphenyl + H(+) + NADH + O2 = (2R,3S)-3-phenylcyclohexa-3,5-
CC         diene-1,2-diol + NAD(+); Xref=Rhea:RHEA:18165, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:17097, ChEBI:CHEBI:32922,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.12.18;
CC   -!- PATHWAY: Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-
CC       pentadienoate and benzoate from biphenyl: step 1/4.
CC   -!- SUBUNIT: Heterohexamer consisting of 3 BphA1 subunits and 3 BphA2
CC       subunits. A ferredoxin (BphA3) and a ferredoxin reductase (BphA4) must
CC       be present to obtain activity.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       beta subunit family. {ECO:0000305}.
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DR   EMBL; D17319; BAA04138.1; -; Genomic_DNA.
DR   PIR; JC2439; JC2439.
DR   AlphaFoldDB; Q52439; -.
DR   SMR; Q52439; -.
DR   UniPathway; UPA00155; UER00250.
DR   GO; GO:0018687; F:biphenyl 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   CDD; cd00667; ring_hydroxylating_dioxygenases_beta; 1.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   PANTHER; PTHR41534; PTHR41534; 1.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; NAD; Oxidoreductase.
FT   CHAIN           1..193
FT                   /note="Biphenyl dioxygenase subunit beta"
FT                   /id="PRO_0000085070"
SQ   SEQUENCE   193 AA;  22779 MW;  5407874F7003A8A4 CRC64;
     MTRVAMAQKT AELIQEFAWP AQPVSPQLQH EVEQFYYREA QLLDHHNYNA WFDLLEQDIH
     YWMPIRTTRI KRENDKEYIP AGANAHFDEN HETMRGRIRQ RTSELNWAED PLSRTRHIVS
     NVIVRETDTP ATLEVASAFL VYRNRLERQV DIFAGERRDV LRRANNGLGF KIARRTILID
     QSTILANNLS VFF