ID   T2M2_AERPE              Reviewed;         156 AA.
AC   Q9YAD8;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Putative type II restriction enzyme ApeKORF2002P {ECO:0000303|PubMed:12654995};
DE            Short=ApeKORF2002P {ECO:0000303|PubMed:12654995};
DE            EC=3.1.21.4 {ECO:0000303|PubMed:12654995};
GN   OrderedLocusNames=APE_2001.1;
OS   Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS   K1).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Aeropyrum.
OX   NCBI_TaxID=272557;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX   PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA   Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA   Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT   "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT   Aeropyrum pernix K1.";
RL   DNA Res. 6:83-101(1999).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A putative type II restriction enzyme, its methylase would be
CC       APE_2002. {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000303|PubMed:12654995};
CC   -!- SIMILARITY: To M.jannaschii MJ1199. {ECO:0000305}.
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DR   EMBL; BA000002; BAA81011.2; -; Genomic_DNA.
DR   PIR; C72503; C72503.
DR   AlphaFoldDB; Q9YAD8; -.
DR   SMR; Q9YAD8; -.
DR   STRING; 272557.APE_2001.1; -.
DR   REBASE; 5093; ApeKORF2002P.
DR   EnsemblBacteria; BAA81011; BAA81011; APE_2001.1.
DR   KEGG; ape:APE_2001.1; -.
DR   PATRIC; fig|272557.25.peg.1335; -.
DR   eggNOG; arCOG01019; Archaea.
DR   Proteomes; UP000002518; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR018665; DUF2122_RecB-nuclease-rel.
DR   Pfam; PF09895; DUF2122; 1.
PE   4: Predicted;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Restriction system.
FT   CHAIN           1..156
FT                   /note="Putative type II restriction enzyme ApeKORF2002P"
FT                   /id="PRO_0000107213"
SQ   SEQUENCE   156 AA;  16177 MW;  1FFB7BCFE5D3E140 CRC64;
     MPVEVIPVLH NVSSVQRVVD MARLSYSLGL DTLVVTKAYG GAAQSGVPEA MRLALKLGKS
     LVVLPELRDA VNLLSPTHVL AVTPSRAERL VGPGGLEGLE GRVLVVFSGG EPELDPSEAA
     GAIRVYIEGV EGKVGPIAEA ALILYFLLRG GGDGRG