ID   T2M2_METJA              Reviewed;         370 AA.
AC   Q58844;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Type II restriction enzyme MjaII {ECO:0000303|PubMed:12654995};
DE            Short=R.MjaII;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease MjaII;
DE   AltName: Full=Type-2 restriction enzyme MjaII;
GN   Name=mjaIIR; OrderedLocusNames=MJ1449;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   CHARACTERIZATION.
RA   Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D.;
RT   "Method for screening restriction endonucleases.";
RL   Patent number WO9911821, 11-MAR-1999.
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGNCC-3'; the cleavage site is unknown.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- SIMILARITY: Belongs to the TdeIII type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99461.1; -; Genomic_DNA.
DR   PIR; H64480; H64480.
DR   RefSeq; WP_010870969.1; NC_000909.1.
DR   AlphaFoldDB; Q58844; -.
DR   STRING; 243232.MJ_1449; -.
DR   REBASE; 1222; MjaII.
DR   EnsemblBacteria; AAB99461; AAB99461; MJ_1449.
DR   GeneID; 1452353; -.
DR   KEGG; mja:MJ_1449; -.
DR   eggNOG; arCOG07628; Archaea.
DR   HOGENOM; CLU_756000_0_0_2; -.
DR   InParanoid; Q58844; -.
DR   OMA; PNAGEMR; -.
DR   OrthoDB; 58569at2157; -.
DR   PRO; PR:Q58844; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019045; Restrct_endonuc_II_TdeIII.
DR   Pfam; PF09520; RE_TdeIII; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Restriction system.
FT   CHAIN           1..370
FT                   /note="Type II restriction enzyme MjaII"
FT                   /id="PRO_0000077333"
SQ   SEQUENCE   370 AA;  43911 MW;  8B4CF6208F914B33 CRC64;
     MPLSKNVIEK ISIETIRVLK SRFDTISDED IKIRNMPFHM AFLRAFYGKI GINDDTEALK
     FLTLSQWFHG LSTTLGQSYF ENIAHILSNG EKRTFKNYKI KRSVRDKISE IINDLKSGER
     LPNVEKENKE LREATSKNSE YVNGLEFTAD VYFEDKDKVV MIELKTVRPN AGEMRGEKQK
     ILYGKAYMME TKPNKKVYYF IGFPYDPTEN PENPCGYDKD RFMSSLIEFS KYFDKREVLI
     AEELWSFLSG EENTMKKILD IINSIAKPDF KEKFDFINTF PFINQDRLYT KDAIDEQKFK
     KYMDILQEWR LYSEIECAKA VKELSLLKLS SRDRRTFERL INNSMFSNNN KYNENRKMKI
     LELYNKYMQK