ID   T2M3_METJA              Reviewed;         290 AA.
AC   Q58017;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Type II restriction enzyme MjaIII {ECO:0000303|PubMed:12654995};
DE            Short=R.MjaIII;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease MjaIII;
DE   AltName: Full=Type-2 restriction enzyme MjaIII;
GN   Name=mjaIIIR; OrderedLocusNames=MJ0600;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   CHARACTERIZATION.
RA   Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D.;
RT   "Method for screening restriction endonucleases.";
RL   Patent number WO9911821, 11-MAR-1999.
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GATC-3'; the cleavage site is unknown.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98591.1; -; Genomic_DNA.
DR   PIR; H64374; H64374.
DR   RefSeq; WP_010870104.1; NC_000909.1.
DR   AlphaFoldDB; Q58017; -.
DR   SMR; Q58017; -.
DR   STRING; 243232.MJ_0600; -.
DR   REBASE; 3891; MjaIII.
DR   EnsemblBacteria; AAB98591; AAB98591; MJ_0600.
DR   GeneID; 1451465; -.
DR   KEGG; mja:MJ_0600; -.
DR   eggNOG; arCOG06575; Archaea.
DR   HOGENOM; CLU_089327_0_0_2; -.
DR   OMA; GLEQIFC; -.
DR   OrthoDB; 110729at2157; -.
DR   PhylomeDB; Q58017; -.
DR   PRO; PR:Q58017; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR   InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR   Pfam; PF04556; DpnII; 1.
DR   PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Restriction system.
FT   CHAIN           1..290
FT                   /note="Type II restriction enzyme MjaIII"
FT                   /id="PRO_0000077334"
SQ   SEQUENCE   290 AA;  34392 MW;  ACEBAE31B029900D CRC64;
     MNFEYIINSL LETIKTYNFF VDWEKIENNI KKIEKRLHIL NYLIGKENFK EEFFELLKEY
     PEVITVFPIL IAVRDNKITI LNENMELETL EFKEKKYLTD EEIERYYKFF KETGLEDLLK
     NRKIKNLVDY VFGVEVGMDT NARKNRIGDL MENIVKKYIE NLCKQNKNLD YIFQATKDKI
     KQKWGINLTL DKTNRKFDFA VFNKNTKKLY LIEVNFYSGG GSKLKATAGE YRSLNEFIKN
     NNNNVQFIWI TDGKGWNTAK NPLKESFNSG VVILNLKMVK EGLLKEILTQ