ID   T2M5_METJA              Reviewed;         230 AA.
AC   Q58895;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Type II restriction enzyme MjaV {ECO:0000303|PubMed:12654995};
DE            Short=R.MjaV;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease MjaV;
DE   AltName: Full=Type-2 restriction enzyme MjaV;
GN   Name=mjaVR; OrderedLocusNames=MJ1500;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   CHARACTERIZATION.
RA   Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D.;
RT   "Method for screening restriction endonucleases.";
RL   Patent number WO9911821, 11-MAR-1999.
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GTAC-3'; the cleavage site is unknown.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; L77117; AAB99521.1; -; Genomic_DNA.
DR   PIR; C64487; C64487.
DR   RefSeq; WP_010871023.1; NC_000909.1.
DR   AlphaFoldDB; Q58895; -.
DR   STRING; 243232.MJ_1500; -.
DR   REBASE; 3898; MjaV.
DR   EnsemblBacteria; AAB99521; AAB99521; MJ_1500.
DR   GeneID; 1452407; -.
DR   KEGG; mja:MJ_1500; -.
DR   eggNOG; arCOG08288; Archaea.
DR   HOGENOM; CLU_104917_0_0_2; -.
DR   OMA; HRTFYAS; -.
DR   OrthoDB; 66837at2157; -.
DR   BRENDA; 3.1.21.4; 3260.
DR   PRO; PR:Q58895; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Restriction system.
FT   CHAIN           1..230
FT                   /note="Type II restriction enzyme MjaV"
FT                   /id="PRO_0000077336"
SQ   SEQUENCE   230 AA;  26764 MW;  9566FE2061C144D5 CRC64;
     MDDKSYYEEI ESILRQILQP IEKISFSTFI RVVSGYKIIP IDLSKKEDKE LINDLAKACN
     EVIEEIKKTG GVKTKEGKTP KRVNEVGNHI EHYVKDVLNK YGYAITPKTK KGKQKSTGYP
     DIEFWYKGKK ERDGRVVYIE IKTFNEKNIN SSHRTFYASP SKDEEGVKIR YDAPHLCLSF
     KIEKLGRDYY ATGFKIIDLS KLKGGIKREF NASNRELYKK DLIIYEKDLK