T2M6_METJA
ID T2M6_METJA Reviewed; 226 AA.
AC P81326;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Putative type II restriction enzyme MjaVIP {ECO:0000303|PubMed:12654995};
DE Short=R.MjaVIP;
DE EC=3.1.21.4;
DE AltName: Full=Endonuclease MjaVIP;
DE AltName: Full=Type-2 restriction enzyme MjaVIP;
GN Name=mjaVIRP; OrderedLocusNames=MJ1208.1;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded sequence 5'-CCGG-3'; the cleavage site is unknown.
CC {ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC -!- SIMILARITY: Belongs to the BsaWI type II restriction endonuclease
CC family. {ECO:0000305}.
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DR EMBL; L77117; AAB99221.1; -; Genomic_DNA.
DR RefSeq; WP_010870721.1; NC_000909.1.
DR AlphaFoldDB; P81326; -.
DR REBASE; 16564; MjaVIP.
DR PRIDE; P81326; -.
DR EnsemblBacteria; AAB99221; AAB99221; MJ_1208.1.
DR GeneID; 27929990; -.
DR KEGG; mja:MJ_1208.1; -.
DR eggNOG; arCOG00115; Archaea.
DR HOGENOM; CLU_095647_0_0_2; -.
DR InParanoid; P81326; -.
DR OMA; AKCINGE; -.
DR OrthoDB; 133558at2157; -.
DR PhylomeDB; P81326; -.
DR PRO; PR:P81326; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR InterPro; IPR041551; RE_BsaWI.
DR Pfam; PF18643; RE_BsaWI; 1.
PE 3: Inferred from homology;
KW DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW Restriction system.
FT CHAIN 1..226
FT /note="Putative type II restriction enzyme MjaVIP"
FT /id="PRO_0000077337"
SQ SEQUENCE 226 AA; 26711 MW; 9A7B306A28A6A2F2 CRC64;
MLHFGGFIME INHISKILEK EREEYIRNKV EEYLKQGFSK DDAVNKANQS WRTYIGHRIQ
DVIYNLLKKF LKDSGLKVTT DKALNNRNLP EELDKVKRLI AINYGEYLFL PDADVIVYKV
ENNDIKIIAI ISVKNSFRER RFETTYWKLK LKESPVTSHI KVFLATPDKD NEISYKCPNG
KPKKMRIILE YELDGIYFLK EDFEETEKAK HFGKIVEDII EISKKL