ID   T2MT_METTF              Reviewed;         281 AA.
AC   P29565;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Type II restriction enzyme MthTI {ECO:0000303|PubMed:12654995};
DE            Short=R.MthTI {ECO:0000303|PubMed:1512204};
DE            EC=3.1.21.4 {ECO:0000269|PubMed:12654995};
DE   AltName: Full=Endonuclease MthTI;
DE   AltName: Full=Type-2 restriction enzyme MthTI;
GN   Name=mthTIR {ECO:0000303|PubMed:1512204};
OS   Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum).
OG   Plasmid pFV1.
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=145262;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=DSM 3848 / THF;
RX   PubMed=1512204; DOI=10.1128/jb.174.17.5719-5726.1992;
RA   Noelling J., de Vos W.M.;
RT   "Characterization of the archaeal, plasmid-encoded type II restriction-
RT   modification system MthTI from Methanobacterium thermoformicicum THF:
RT   homology to the bacterial NgoPII system from Neisseria gonorrhoeae.";
RL   J. Bacteriol. 174:5719-5726(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 3848 / THF;
RX   PubMed=1336177; DOI=10.1093/nar/20.24.6501;
RA   Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.;
RT   "Modular organization of related Archaeal plasmids encoding different
RT   restriction-modification systems in Methanobacterium thermoformicicum.";
RL   Nucleic Acids Res. 20:6501-6507(1992).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGCC-3' and cleaves after G-1.
CC       {ECO:0000269|PubMed:1512204, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:1512204};
CC   -!- SIMILARITY: Belongs to the NgoPII type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; M97222; AAA73369.1; -; Genomic_DNA.
DR   EMBL; X68366; CAA48435.1; -; Genomic_DNA.
DR   PIR; A42941; A42941.
DR   RefSeq; NP_039764.1; NC_001336.1.
DR   RefSeq; WP_010889850.1; NC_001336.1.
DR   AlphaFoldDB; P29565; -.
DR   REBASE; 1284; MthTI.
DR   PRO; PR:P29565; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019046; Restrct_endonuc_II_NgoPII.
DR   Pfam; PF09521; RE_NgoPII; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Restriction system.
FT   CHAIN           1..281
FT                   /note="Type II restriction enzyme MthTI"
FT                   /id="PRO_0000077339"
SQ   SEQUENCE   281 AA;  32102 MW;  083200CE4A7CD49A CRC64;
     MSNLLQAIMN IKSIQERNLG SYKGVQDSKN RMNQMGVTLE VFLKDAFCNT FDIENKDLVY
     SEYFSYLGNQ NNPPDMILKG GDAVEVKKIT GIKTSIQLNS SYPKSKLFVS DSRITEACKN
     CEDWEVKDII YAIGTIPNRV LKLMFFVYGD CYAASPSIYQ RIVEEVKGGL HSTGLEFSET
     NELGRINRVD PLGITDLRVR GMWIIKHPIK VFKNIIPPES IKNNNFNLIA LMKAEKYKQF
     PKKDRKRIEA EDNIEVTDVK IKDPDNPAKL LDSVLVRYDE I