T2MW_METWO
ID T2MW_METWO Reviewed; 363 AA.
AC O59646;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=Type II restriction enzyme MwoI {ECO:0000303|PubMed:12654995};
DE Short=R.MwoI {ECO:0000303|PubMed:2663652};
DE EC=3.1.21.4 {ECO:0000269|PubMed:2663652};
DE AltName: Full=Endonuclease MwoI;
DE AltName: Full=Type-2 restriction enzyme MwoI;
GN Name=mwoIR {ECO:0000303|PubMed:2663652};
OS Methanothermobacter wolfeii (Methanobacterium wolfei).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=145261;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829;
RA Lunnen K.D., Wilson G.G.;
RT "Method for producing the MwoI restriction endonuclease and methylase.";
RL Patent number US5053330, 01-OCT-1991.
RN [2]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829;
RX PubMed=2663652; DOI=10.1016/0378-1119(89)90354-5;
RA Lunnen K.D., Morgan R.D., Timan C.J., Krzycki J.A., Reeve J.N.,
RA Wilson G.G.;
RT "Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-
RT modification system from Methanobacterium wolfei.";
RL Gene 77:11-19(1989).
RN [3]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded sequence 5'-GCNNNNNNNGC-3' and cleaves after N-5.
CC {ECO:0000269|PubMed:2663652}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC Evidence={ECO:0000269|PubMed:2663652};
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DR EMBL; AF051376; AAC05699.1; -; Genomic_DNA.
DR AlphaFoldDB; O59646; -.
DR REBASE; 1291; MwoI.
DR PRO; PR:O59646; -.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Endonuclease; Hydrolase; Nuclease; Restriction system.
FT CHAIN 1..363
FT /note="Type II restriction enzyme MwoI"
FT /id="PRO_0000077342"
SQ SEQUENCE 363 AA; 42214 MW; 5CC35411A18028A4 CRC64;
MKNPKFTENQ KEIEKEEFEF LQKLNFIIKE SLELFNTNLK NSMKFINYIT LPIIMASIES
KSFNPFSEII EKHIAFILNS KMNSLGYKFL PLGYSSDLTY ENDNSIIHID IKTANLENPS
DFKDTVPLGI NQSSYPGVLD CKIRGKNIKA DCKKIKVYPN IPTTYNNKLT ITNALLFIYP
DYKEIIDEIR EDYIAIRELI SINLKDILTP IEGSLEEFLN YKPSNEKKRL EPILDNIVRG
YFIHDKLRHE FSENVEKDLE EFEKKIIGIA KKLKEREIKP VAILSISIPN GELAPHYDDE
IVSGKSWGSS FRYHYKKSGN SVFKGLDNKA SRAVFLHINK EYLPVLKKYF DPITVYELTE
KRL