ID   T2MZ_METTF              Reviewed;         202 AA.
AC   P29566;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Type II restriction enzyme MthZI {ECO:0000303|PubMed:12654995};
DE            Short=R.MthZI {ECO:0000303|PubMed:1408820};
DE            EC=3.1.21.4 {ECO:0000269|PubMed:1408820};
DE   AltName: Full=Endonuclease MthZI;
DE   AltName: Full=Type-2 restriction enzyme MthZI;
GN   Name=mthZIR {ECO:0000303|PubMed:1408820};
OS   Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum).
OG   Plasmid pFZ1.
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=145262;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=DSM 3720 / Z-245;
RX   PubMed=1408820; DOI=10.1093/nar/20.19.5047;
RA   Noelling J., de Vos W.M.;
RT   "Identification of the CTAG-recognizing restriction-modification systems
RT   MthZI and MthFI from Methanobacterium thermoformicicum and characterization
RT   of the plasmid-encoded mthZIM gene.";
RL   Nucleic Acids Res. 20:5047-5052(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 3720 / Z-245;
RX   PubMed=1336177; DOI=10.1093/nar/20.24.6501;
RA   Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.;
RT   "Modular organization of related Archaeal plasmids encoding different
RT   restriction-modification systems in Methanobacterium thermoformicicum.";
RL   Nucleic Acids Res. 20:6501-6507(1992).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CTAG-3' and cleaves after C-1.
CC       {ECO:0000269|PubMed:1408820, ECO:0000303|PubMed:1336177}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:1408820};
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DR   EMBL; X67212; CAA47652.1; -; Genomic_DNA.
DR   EMBL; X68367; CAA48446.1; -; Genomic_DNA.
DR   PIR; S26074; S26074.
DR   RefSeq; NP_039774.1; NC_001337.1.
DR   RefSeq; WP_010889861.1; NC_001337.1.
DR   AlphaFoldDB; P29566; -.
DR   REBASE; 2204; MthZI.
DR   GeneID; 24855016; -.
DR   PRO; PR:P29566; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019068; Restrct_endonuc_II_MjaI.
DR   Pfam; PF09568; RE_MjaI; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Restriction system.
FT   CHAIN           1..202
FT                   /note="Type II restriction enzyme MthZI"
FT                   /id="PRO_0000077340"
SQ   SEQUENCE   202 AA;  23742 MW;  1AC0274DEC882F49 CRC64;
     MIDNFKEIRL DFKDELKKIT GKEVEFPKYT TQIINLANQN AQGTRPRVVG QMSDLIHECP
     DKSYEGWKKW YLEHYSDRIE KATKKISKMI ENMKAAMELI DEEMIRKWVE DLVITKTAEG
     LIIQEIILKT IAEEAGLEWR LATSKEESKN IDGFIGSTPV SIKPMSYESM RPTVREEIDI
     QTIFYKKPKN SRYLYIYHNL NI