ID   T2N5_NOSS7              Reviewed;         220 AA.
AC   P35677; K9QQL2;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 2.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Type II restriction enzyme NspV {ECO:0000303|PubMed:12654995};
DE            Short=R.NspV {ECO:0000303|PubMed:8367311};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease NspV;
DE   AltName: Full=Type-2 restriction enzyme NspV;
GN   Name=nspVR {ECO:0000303|PubMed:8367311}; OrderedLocusNames=Nos7524_1225;
OS   Nostoc sp. (strain ATCC 29411 / PCC 7524).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=28072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PROTEIN SEQUENCE OF
RP   N-TERMINUS.
RC   STRAIN=ATCC 29411 / PCC 7524;
RX   PubMed=8367311; DOI=10.1093/nar/21.16.3899;
RA   Ueno T., Ito H., Kotani H., Nakajima K.;
RT   "Cloning and expression of the NspV restriction-modification genes of
RT   Nostoc sp. strain PCC7524.";
RL   Nucleic Acids Res. 21:3899-3899(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29411 / PCC 7524;
RX   PubMed=23277585; DOI=10.1073/pnas.1217107110;
RA   Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A.,
RA   Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K.,
RA   Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S.,
RA   Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.;
RT   "Improving the coverage of the cyanobacterial phylum using diversity-driven
RT   genome sequencing.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-TTCGAA-3' and cleaves after T-2.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8367311}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; D14719; BAA03538.1; -; Genomic_DNA.
DR   EMBL; CP003552; AFY47112.1; -; Genomic_DNA.
DR   RefSeq; WP_015137568.1; NC_019684.1.
DR   AlphaFoldDB; P35677; -.
DR   STRING; 28072.Nos7524_1225; -.
DR   REBASE; 1407; NspV.
DR   REBASE; 57743; Nsp7524ORF1224P.
DR   EnsemblBacteria; AFY47112; AFY47112; Nos7524_1225.
DR   KEGG; nop:Nos7524_1225; -.
DR   PATRIC; fig|28072.8.peg.1340; -.
DR   eggNOG; COG0827; Bacteria.
DR   HOGENOM; CLU_110623_0_0_3; -.
DR   OMA; HKFRAYL; -.
DR   OrthoDB; 1229785at2; -.
DR   BRENDA; 3.1.21.4; 4371.
DR   PRO; PR:P35677; -.
DR   Proteomes; UP000010378; Chromosome.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome; Restriction system.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8367311"
FT   CHAIN           2..220
FT                   /note="Type II restriction enzyme NspV"
FT                   /id="PRO_0000077352"
FT   CONFLICT        175..177
FT                   /note="DEI -> AIL (in Ref. 1; BAA03538)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   220 AA;  24664 MW;  D788B9605C0ED16B CRC64;
     MTILTIEALR TEAAIFSAAE SIHPEPLLYG VTDGKAVGTY IEQKFRLYLK EHYEFVQGNS
     ASGIDFPGLL VDVKVTSIRQ PQSSCPFKSA RQKIFGLGYS LLIFVYDKID NSTNRTATLN
     ILHTIYVSAE RTADFQMTRG IRNILANEGN KDDLIAFMSD RNLPVDEIEA GNVADEILRN
     PPMQGFLTIS NALQWRLQYG RVIERAGQED GILTVYRNNP