ID   T2P1_PROHU              Reviewed;          11 AA.
AC   P31031;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   23-FEB-2022, entry version 52.
DE   RecName: Full=Type II restriction enzyme PvuI {ECO:0000303|PubMed:12654995};
DE            Short=R.PvuI {ECO:0000303|PubMed:1454536};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease PvuI;
DE   AltName: Full=Type-2 restriction enzyme PvuI;
DE   Flags: Fragment;
GN   Name=pvuIR;
OS   Proteus hauseri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Proteus.
OX   NCBI_TaxID=183417;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 13315 / DSM 30118 / JCM 1668 / NBRC 3851 / NCIMB 4175 / NCTC
RC   4175 / NRRL B-3405;
RX   PubMed=1454536; DOI=10.1093/nar/20.21.5743;
RA   Smith M.D., Longo M., Gerard G.F., Chatterjee D.K.;
RT   "Cloning and characterization of genes for the PvuI restriction and
RT   modification system.";
RL   Nucleic Acids Res. 20:5743-5747(1992).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CGATCG-3' and cleaves after T-4.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:1454536}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; L04163; AAA25660.1; -; Genomic_DNA.
DR   PIR; S35490; S35490.
DR   REBASE; 1541; PvuI.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           <1..11
FT                   /note="Type II restriction enzyme PvuI"
FT                   /id="PRO_0000077355"
FT   NON_TER         1
SQ   SEQUENCE   11 AA;  1300 MW;  9F0CDE7955B72B1A CRC64;
     ISVDELSNYN F