ID   T2P2_NEIGO              Reviewed;         278 AA.
AC   P24617;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Type II restriction enzyme NgoPII {ECO:0000303|PubMed:12654995};
DE            Short=R.NgoPII {ECO:0000303|PubMed:2501649};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease NgoPII;
DE   AltName: Full=Type-2 restriction enzyme NgoPII;
GN   Name=ngoPIIR; Synonyms=dcrB;
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=P9;
RX   PubMed=2501649; DOI=10.1007/bf00334379;
RA   Sullivan K.M., Saunders J.R.;
RT   "Nucleotide sequence and genetic organization of the NgoPII restriction-
RT   modification system of Neisseria gonorrhoeae.";
RL   Mol. Gen. Genet. 216:380-387(1989).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGCC-3' and cleaves after G-2.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- SIMILARITY: Belongs to the NgoPII type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; X52661; CAA36887.1; -; Genomic_DNA.
DR   PIR; S04419; S04419.
DR   RefSeq; WP_003690022.1; NZ_VAHL01000002.1.
DR   AlphaFoldDB; P24617; -.
DR   REBASE; 1331; NgoPII.
DR   PRO; PR:P24617; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019046; Restrct_endonuc_II_NgoPII.
DR   Pfam; PF09521; RE_NgoPII; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..278
FT                   /note="Type II restriction enzyme NgoPII"
FT                   /id="PRO_0000077348"
SQ   SEQUENCE   278 AA;  31501 MW;  1BF8B25BEEC96903 CRC64;
     MNIIDAIINL ANNPVVGVES HSQSNNRANQ AGDALEEYVK DLFSGSFNLN ETQRIARHAK
     VFSYLGNNSN PPDAMLRNGD AIEVKKIESK DSALALNSSH PKSKLSVDDS MLTKACKDAE
     KWEEKDIIYI VGVVDKKKNL KHLAMVYGID YCADAECYLK IKNQIKEGIG NIGGIQFAET
     KELGRVNRID PLNITYLRVR GMWGIENPWF VFNYIYQRNM EKSFNFMAII NEDKWNSFNN
     TDKLLAIQDS KLAISDIKIK NPNNPARLRN AKLITYHL