T2R14_HUMAN
ID T2R14_HUMAN Reviewed; 317 AA.
AC Q9NYV8; Q645X3;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Taste receptor type 2 member 14;
DE Short=T2R14;
DE AltName: Full=Taste receptor family B member 1;
DE Short=TRB1;
GN Name=TAS2R14;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY.
RX PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9;
RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA Zuker C.S.;
RT "A novel family of mammalian taste receptors.";
RL Cell 100:693-702(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15496549; DOI=10.1093/molbev/msi027;
RA Fischer A., Gilad Y., Man O., Paeaebo S.;
RT "Evolution of bitter taste receptors in humans and apes.";
RL Mol. Biol. Evol. 22:432-436(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP CHARACTERIZATION.
RX PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0;
RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA Zuker C.S., Ryba N.J.P.;
RT "T2Rs function as bitter taste receptors.";
RL Cell 100:703-711(2000).
RN [5]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [6]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [7]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
RN [8]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-153.
RC TISSUE=Plasma;
RX PubMed=16335952; DOI=10.1021/pr0502065;
RA Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA Smith R.D.;
RT "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT hydrazide chemistry, and mass spectrometry.";
RL J. Proteome Res. 4:2070-2080(2005).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=16720576; DOI=10.1074/jbc.m513637200;
RA Behrens M., Bartelt J., Reichling C., Winnig M., Kuhn C., Meyerhof W.;
RT "Members of RTP and REEP gene families influence functional bitter taste
RT receptor expression.";
RL J. Biol. Chem. 281:20650-20659(2006).
CC -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC and is gustducin-linked. May play a role in sensing the chemical
CC composition of the gastrointestinal content. The activity of this
CC receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC and lead to the gating of TRPM5 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and palate epithelium and exclusively in gustducin-positive
CC cells. Expressed in testis (PubMed:16720576).
CC {ECO:0000269|PubMed:16720576}.
CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC bitter compounds; individual taste cells can discriminate among bitter
CC stimuli.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF227138; AAF43911.1; -; Genomic_DNA.
DR EMBL; AY724944; AAU21146.1; -; Genomic_DNA.
DR EMBL; BC069148; AAH69148.1; -; mRNA.
DR EMBL; BC101993; AAI01994.1; -; mRNA.
DR EMBL; BC101994; AAI01995.1; -; mRNA.
DR EMBL; BC103699; AAI03700.1; -; mRNA.
DR CCDS; CCDS8637.1; -.
DR RefSeq; NP_076411.1; NM_023922.1.
DR AlphaFoldDB; Q9NYV8; -.
DR SMR; Q9NYV8; -.
DR BioGRID; 119150; 9.
DR STRING; 9606.ENSP00000441949; -.
DR BindingDB; Q9NYV8; -.
DR ChEMBL; CHEMBL3309105; -.
DR DrugCentral; Q9NYV8; -.
DR GlyGen; Q9NYV8; 3 sites.
DR iPTMnet; Q9NYV8; -.
DR PhosphoSitePlus; Q9NYV8; -.
DR BioMuta; TAS2R14; -.
DR DMDM; 29839658; -.
DR PaxDb; Q9NYV8; -.
DR PeptideAtlas; Q9NYV8; -.
DR PRIDE; Q9NYV8; -.
DR Antibodypedia; 23413; 88 antibodies from 16 providers.
DR DNASU; 50840; -.
DR Ensembl; ENST00000537503.2; ENSP00000441949.1; ENSG00000212127.6.
DR Ensembl; ENST00000575008.1; ENSP00000458544.1; ENSG00000261984.1.
DR Ensembl; ENST00000614274.2; ENSP00000484251.1; ENSG00000276541.2.
DR GeneID; 50840; -.
DR KEGG; hsa:50840; -.
DR MANE-Select; ENST00000537503.2; ENSP00000441949.1; NM_023922.2; NP_076411.1.
DR UCSC; uc010shi.3; human.
DR CTD; 50840; -.
DR DisGeNET; 50840; -.
DR GeneCards; TAS2R14; -.
DR HGNC; HGNC:14920; TAS2R14.
DR HPA; ENSG00000212127; Low tissue specificity.
DR MIM; 604790; gene.
DR neXtProt; NX_Q9NYV8; -.
DR OpenTargets; ENSG00000212127; -.
DR PharmGKB; PA37930; -.
DR VEuPathDB; HostDB:ENSG00000212127; -.
DR eggNOG; ENOG502SKRK; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_2_0_1; -.
DR InParanoid; Q9NYV8; -.
DR OMA; IHINASI; -.
DR OrthoDB; 1053374at2759; -.
DR PhylomeDB; Q9NYV8; -.
DR TreeFam; TF335891; -.
DR PathwayCommons; Q9NYV8; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 50840; 14 hits in 1074 CRISPR screens.
DR GeneWiki; TAS2R14; -.
DR GenomeRNAi; 50840; -.
DR Pharos; Q9NYV8; Tchem.
DR PRO; PR:Q9NYV8; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9NYV8; protein.
DR Bgee; ENSG00000212127; Expressed in corpus callosum and 96 other tissues.
DR Genevisible; Q9NYV8; HS.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; IDA:HGNC-UCL.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IC:HGNC-UCL.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 1: Evidence at protein level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..317
FT /note="Taste receptor type 2 member 14"
FT /id="PRO_0000082255"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..87
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..184
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..261
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:16335952"
FT CARBOHYD 162
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 86
FT /note="T -> A (in dbSNP:rs16925868)"
FT /id="VAR_053347"
FT VARIANT 201
FT /note="L -> F (in dbSNP:rs35804287)"
FT /id="VAR_062085"
SQ SEQUENCE 317 AA; 36160 MW; C79184343D679BE5 CRC64;
MGGVIKSIFT FVLIVEFIIG NLGNSFIALV NCIDWVKGRK ISSVDRILTA LAISRISLVW
LIFGSWCVSV FFPALFATEK MFRMLTNIWT VINHFSVWLA TGLGTFYFLK IANFSNSIFL
YLKWRVKKVV LVLLLVTSVF LFLNIALINI HINASINGYR RNKTCSSDSS NFTRFSSLIV
LTSTVFIFIP FTLSLAMFLL LIFSMWKHRK KMQHTVKISG DASTKAHRGV KSVITFFLLY
AIFSLSFFIS VWTSERLEEN LIILSQVMGM AYPSCHSCVL ILGNKKLRQA SLSVLLWLRY
MFKDGEPSGH KEFRESS
