T2R16_MOUSE
ID T2R16_MOUSE Reviewed; 299 AA.
AC P59529; Q7M728;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Taste receptor type 2 member 16;
DE Short=T2R16;
DE AltName: Full=Candidate taste receptor mt2r40;
DE AltName: Full=T2R18;
GN Name=Tas2r16; Synonyms=Tas2r118, Tas2r18;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Zuker C.S., Adler J.E., Ryba N., Mueller K., Hoon M.;
RT "T2r taste receptor family.";
RL Patent number WO0118050, 15-MAR-2001.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION.
RX PubMed=12679530; DOI=10.1093/molbev/msg083;
RA Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT "Adaptive diversification of bitter taste receptor genes in mammalian
RT evolution.";
RL Mol. Biol. Evol. 20:805-814(2003).
RN [5]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [6]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [7]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Gustducin-coupled receptor implicated in the perception of
CC bitter compounds in the oral cavity and the gastrointestinal tract.
CC Signals through PLCB2 and the calcium-regulated cation channel TRPM5
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with RTP3 and RTP4. {ECO:0000250|UniProtKB:Q9NYV7}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9NYV7};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AX097834; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC153637; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC104401; AAI04402.1; -; mRNA.
DR EMBL; BK001069; DAA01208.1; -; Genomic_DNA.
DR CCDS; CCDS19939.1; -.
DR RefSeq; NP_996905.1; NM_207022.1.
DR AlphaFoldDB; P59529; -.
DR SMR; P59529; -.
DR STRING; 10090.ENSMUSP00000053700; -.
DR GlyGen; P59529; 1 site.
DR PhosphoSitePlus; P59529; -.
DR PaxDb; P59529; -.
DR PRIDE; P59529; -.
DR Antibodypedia; 31744; 66 antibodies from 16 providers.
DR DNASU; 387347; -.
DR Ensembl; ENSMUST00000062463; ENSMUSP00000053700; ENSMUSG00000043865.
DR GeneID; 387347; -.
DR KEGG; mmu:387347; -.
DR UCSC; uc009bbn.1; mouse.
DR CTD; 387347; -.
DR MGI; MGI:2681247; Tas2r118.
DR VEuPathDB; HostDB:ENSMUSG00000043865; -.
DR eggNOG; ENOG502S2SI; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_1_1_1; -.
DR InParanoid; P59529; -.
DR OMA; CLQWTSM; -.
DR OrthoDB; 1282504at2759; -.
DR PhylomeDB; P59529; -.
DR TreeFam; TF335891; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-MMU-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 387347; 1 hit in 71 CRISPR screens.
DR PRO; PR:P59529; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P59529; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; ISO:MGI.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISO:MGI.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Taste; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..299
FT /note="Taste receptor type 2 member 16"
FT /id="PRO_0000082267"
FT TOPO_DOM 1..5
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..82
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..183
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..258
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 299 AA; 34761 MW; 535B13833919517C CRC64;
MVPTQVTIFS IIMYVLESLV IIVQSCTTVA VLFREWMHFQ RLSPVETILI SLGISHFCLQ
WTSMLYNFGT YSRPVLLFWK VSVVWEFMNI LTFWLTSWLA VLYCVKVSSF THPIFLWLRM
KILKLVLWLI LGALIASCLS IIPSVVKYHI QMELVTLDNL PKNNSLILRL QQFEWYFSNP
LKMIGFGIPF FVFLASIILL TVSLVQHWVQ MKHYSSSNSS LKAQFTVLKS LATFFTFFTS
YFLTIVISFI GTVFDKKSWF WVCEAVIYGL VCIHFTSLMM SNPALKKALK LQFWSPEPS
