T2R16_RAT
ID T2R16_RAT Reviewed; 299 AA.
AC Q9JKU0;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Taste receptor type 2 member 16;
DE Short=T2R16;
DE AltName: Full=T2R18;
DE AltName: Full=T2R3;
GN Name=Tas2r16; Synonyms=Tas2r18, Tas2r3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TOPOLOGY.
RX PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9;
RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA Zuker C.S.;
RT "A novel family of mammalian taste receptors.";
RL Cell 100:693-702(2000).
RN [2]
RP CHARACTERIZATION.
RX PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0;
RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA Zuker C.S., Ryba N.J.P.;
RT "T2Rs function as bitter taste receptors.";
RL Cell 100:703-711(2000).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=11854532; DOI=10.1073/pnas.042617699;
RA Wu S.V., Rozengurt N., Yang M., Young S.H., Sinnett-Smith J., Rozengurt E.;
RT "Expression of bitter taste receptors of the T2R family in the
RT gastrointestinal tract and enteroendocrine STC-1 cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:2392-2397(2002).
RN [4]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [5]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [6]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Gustducin-coupled receptor implicated in the perception of
CC bitter compounds in the oral cavity and the gastrointestinal tract.
CC Signals through PLCB2 and the calcium-regulated cation channel TRPM5.
CC -!- SUBUNIT: Interacts with RTP3 and RTP4. {ECO:0000250|UniProtKB:Q9NYV7}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9NYV7};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and palate epithelium and exclusively in gustducin-positive
CC cells. Expressed in the antrum and fundus (part of the stomach),
CC duodenum and in gastric endocrine cells. {ECO:0000269|PubMed:11854532}.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AF227141; AAF43914.1; -; mRNA.
DR RefSeq; NP_076484.1; NM_023994.1.
DR AlphaFoldDB; Q9JKU0; -.
DR SMR; Q9JKU0; -.
DR STRING; 10116.ENSRNOP00000031530; -.
DR GlyGen; Q9JKU0; 1 site.
DR PhosphoSitePlus; Q9JKU0; -.
DR PaxDb; Q9JKU0; -.
DR Ensembl; ENSRNOT00000034936; ENSRNOP00000031530; ENSRNOG00000021799.
DR GeneID; 78980; -.
DR KEGG; rno:78980; -.
DR UCSC; RGD:620735; rat.
DR CTD; 387347; -.
DR RGD; 620735; Tas2r16.
DR eggNOG; ENOG502S2SI; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_1_1_1; -.
DR InParanoid; Q9JKU0; -.
DR OMA; CLQWTSM; -.
DR OrthoDB; 1282504at2759; -.
DR PhylomeDB; Q9JKU0; -.
DR TreeFam; TF335891; -.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-RNO-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR PRO; PR:Q9JKU0; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Genevisible; Q9JKU0; RN.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:RGD.
DR GO; GO:0009897; C:external side of plasma membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; ISO:RGD.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:RGD.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISO:RGD.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Taste; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..299
FT /note="Taste receptor type 2 member 16"
FT /id="PRO_0000082268"
FT TOPO_DOM 1..5
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..82
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..183
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..258
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 299 AA; 34642 MW; ECDC2EBB29943B6E CRC64;
MVPTQVTIFS IIMYVLESLV IIVQSCTTVA VLFREWMHFQ RLSPVEIILI SLGISHFCLQ
WTSMLYNFGT YSRPVLLFWK VSVVWEFMNV LTFWLTSLLA VLYCVKVSSF SHPVFLWLRL
KILKLVLWLL LGALIASCLS IIPSVVKYHI QMELLTLDHL PKNSSLILRL QMFEWYFSNP
FKMIGFGVPF LVFLISIILL TVSLVQHWGQ MKHYSSSSSS LRAQCTVLKS LATFFIFFTS
YFLTIVVSFI GTVFDKKSWF WVCEAVIYGL VCIHFTSLMM SNPTLKKALR LQFWSPESS
