T2R20_HUMAN
ID T2R20_HUMAN Reviewed; 309 AA.
AC P59543; P59549; Q2HIZ4; Q496D8; Q645X9;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Taste receptor type 2 member 20;
DE AltName: Full=Taste receptor type 2 member 49;
DE Short=T2R49;
DE AltName: Full=Taste receptor type 2 member 56;
DE Short=T2R56;
GN Name=TAS2R20; Synonyms=TAS2R49;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12379855; DOI=10.1038/ng1014;
RA Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.;
RT "The human TAS2R16 receptor mediates bitter taste in response to beta-
RT glucopyranosides.";
RL Nat. Genet. 32:397-401(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12584440; DOI=10.1159/000068546;
RA Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.;
RT "Identification and characterization of human taste receptor genes
RT belonging to the TAS2R family.";
RL Cytogenet. Genome Res. 98:45-53(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ASN-148; VAL-236; SER-252
RP AND LEU-255.
RX PubMed=15496549; DOI=10.1093/molbev/msi027;
RA Fischer A., Gilad Y., Man O., Paeaebo S.;
RT "Evolution of bitter taste receptors in humans and apes.";
RL Mol. Biol. Evol. 22:432-436(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-79.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [6]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [7]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC and is gustducin-linked. May play a role in sensing the chemical
CC composition of the gastrointestinal content. The activity of this
CC receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC and lead to the gating of TRPM5 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and exclusively in gustducin-positive cells.
CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC bitter compounds; individual taste cells can discriminate among bitter
CC stimuli.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AF494236; AAM19327.1; -; Genomic_DNA.
DR EMBL; AY114092; AAM63542.1; -; Genomic_DNA.
DR EMBL; AY724938; AAU21140.1; -; Genomic_DNA.
DR EMBL; BC100915; AAI00916.1; -; mRNA.
DR EMBL; BC100916; AAI00917.1; -; mRNA.
DR EMBL; BC100918; AAI00919.1; -; mRNA.
DR EMBL; BC113843; AAI13844.1; -; mRNA.
DR CCDS; CCDS8639.1; -.
DR RefSeq; NP_795370.2; NM_176889.3.
DR AlphaFoldDB; P59543; -.
DR SMR; P59543; -.
DR BioGRID; 129250; 3.
DR STRING; 9606.ENSP00000441624; -.
DR ChEMBL; CHEMBL3559705; -.
DR GlyGen; P59543; 2 sites.
DR iPTMnet; P59543; -.
DR PhosphoSitePlus; P59543; -.
DR BioMuta; TAS2R20; -.
DR DMDM; 55977811; -.
DR PaxDb; P59543; -.
DR PRIDE; P59543; -.
DR Antibodypedia; 51096; 87 antibodies from 19 providers.
DR DNASU; 259295; -.
DR Ensembl; ENST00000538986.2; ENSP00000441624.1; ENSG00000255837.2.
DR Ensembl; ENST00000570941.1; ENSP00000459353.1; ENSG00000273092.1.
DR GeneID; 259295; -.
DR KEGG; hsa:259295; -.
DR MANE-Select; ENST00000538986.2; ENSP00000441624.1; NM_176889.4; NP_795370.2.
DR UCSC; uc001qzm.3; human.
DR CTD; 259295; -.
DR DisGeNET; 259295; -.
DR GeneCards; TAS2R20; -.
DR HGNC; HGNC:19109; TAS2R20.
DR HPA; ENSG00000255837; Low tissue specificity.
DR MIM; 613962; gene.
DR neXtProt; NX_P59543; -.
DR OpenTargets; ENSG00000255837; -.
DR VEuPathDB; HostDB:ENSG00000255837; -.
DR eggNOG; ENOG502TE6U; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_2_0_1; -.
DR InParanoid; P59543; -.
DR OMA; CWAKGQN; -.
DR OrthoDB; 1339037at2759; -.
DR PhylomeDB; P59543; -.
DR TreeFam; TF335891; -.
DR PathwayCommons; P59543; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 259295; 10 hits in 1002 CRISPR screens.
DR GeneWiki; TAS2R20; -.
DR GenomeRNAi; 259295; -.
DR Pharos; P59543; Tchem.
DR PRO; PR:P59543; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; P59543; protein.
DR Bgee; ENSG00000255837; Expressed in corpus callosum and 93 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0033038; F:bitter taste receptor activity; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..309
FT /note="Taste receptor type 2 member 20"
FT /id="PRO_0000082331"
FT TOPO_DOM 1..6
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..79
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..309
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 79
FT /note="K -> E (in dbSNP:rs7135018)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_053356"
FT VARIANT 143
FT /note="H -> Q (in dbSNP:rs12226920)"
FT /id="VAR_053357"
FT VARIANT 148
FT /note="H -> N (in dbSNP:rs12226919)"
FT /evidence="ECO:0000269|PubMed:15496549"
FT /id="VAR_053358"
FT VARIANT 236
FT /note="I -> V (in dbSNP:rs10845281)"
FT /evidence="ECO:0000269|PubMed:15496549"
FT /id="VAR_053359"
FT VARIANT 252
FT /note="F -> S (in dbSNP:rs10845280)"
FT /evidence="ECO:0000269|PubMed:15496549"
FT /id="VAR_053360"
FT VARIANT 255
FT /note="R -> L (in dbSNP:rs10845279)"
FT /evidence="ECO:0000269|PubMed:15496549"
FT /id="VAR_053361"
FT CONFLICT 33
FT /note="I -> V (in Ref. 3; AAU21140)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="R -> K (in Ref. 1; AAM19327)"
FT /evidence="ECO:0000305"
FT CONFLICT 141..143
FT /note="VCH -> ICQ (in Ref. 3; AAU21140)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 309 AA; 35358 MW; AB200710EE19361C CRC64;
MMSFLHIVFS ILVVVAFILG NFANGFIALI NFIAWVKRQK ISSADQIIAA LAVSRVGLLW
VILLHWYSTV LNPTSSNLKV IIFISNAWAV TNHFSIWLAT SLSIFYLLKI VNFSRLIFHH
LKRKAKSVVL VIVLGSLFFL VCHLVMKHTY INVWTEECEG NVTWKIKLRN AMHLSNLTVA
MLANLIPFTL TLISFLLLIY SLCKHLKKMQ LHGKGSQDPS TKIHIKALQT VTSFLILLAI
YFLCLIISFW NFKMRPKEIV LMLCQAFGII YPSFHSFILI WGNKTLKQTF LSVLWQVTCW
AKGQNQSTP
