T2R40_CHICK
ID T2R40_CHICK Reviewed; 311 AA.
AC Q2AB83; A0A0X9UE61;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Taste receptor type 2 member 40;
DE Short=T2R40;
DE AltName: Full=Taste receptor type 2 member 1;
DE Short=T2R1;
GN Name=TAS2R40; Synonyms=TAS2R1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16484289; DOI=10.1093/molbev/msj106;
RA Go Y.;
RT "Lineage-specific expansions and contractions of the bitter taste receptor
RT gene repertoire in vertebrates.";
RL Mol. Biol. Evol. 23:964-972(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Red jungle fowl;
RX PubMed=15592404; DOI=10.1038/nature03154;
RA Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P.,
RA Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B.,
RA Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C.,
RA Fulton R.S., Graves T.A., Kremitzki C., Layman D., Magrini V.,
RA McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O.,
RA Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W.,
RA Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D.,
RA Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K.,
RA Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L.,
RA Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B.,
RA Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M.,
RA Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K.,
RA Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E.,
RA Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M.,
RA Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M.,
RA Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S.,
RA Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M.,
RA Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M.,
RA Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A.,
RA Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S.,
RA Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J.,
RA Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J.,
RA Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E.,
RA Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M.,
RA Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A.,
RA Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M.,
RA Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O.,
RA Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E.,
RA Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S.,
RA Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E.,
RA Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R.,
RA Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R.,
RA Wilson R.K.;
RT "Sequence and comparative analysis of the chicken genome provide unique
RT perspectives on vertebrate evolution.";
RL Nature 432:695-716(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-297.
RA Su Y., Zhu Q., Wu N., Chen B., Li D.;
RT "The genetic polymorphisms of chicken bitter taste receptor genes in
RT Sichuan domestic and Tibetan populations.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=25796330; DOI=10.1016/j.bbrc.2015.03.056;
RA Hirose N., Kawabata Y., Kawabata F., Nishimura S., Tabata S.;
RT "Bitter taste receptor T2R1 activities were compatible with behavioral
RT sensitivity to bitterness in chickens.";
RL Biochem. Biophys. Res. Commun. 460:464-468(2015).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=26049797; DOI=10.3382/ps/pev152;
RA Cheled-Shoval S.L., Druyan S., Uni Z.;
RT "Bitter, sweet and umami taste receptors and downstream signaling
RT effectors: Expression in embryonic and growing chicken gastrointestinal
RT tract.";
RL Poult. Sci. 94:1928-1941(2015).
RN [6]
RP FUNCTION, AND MUTAGENESIS OF LYS-86; PHE-89; ASN-93; PHE-181; LEU-185;
RP TYR-244; ASN-247 AND LEU-251.
RX PubMed=28811548; DOI=10.1038/s41598-017-08344-9;
RA Di Pizio A., Kruetzfeldt L.M., Cheled-Shoval S., Meyerhof W., Behrens M.,
RA Niv M.Y.;
RT "Ligand binding modes from low resolution GPCR models and mutagenesis:
RT chicken bitter taste receptor as a test-case.";
RL Sci. Rep. 7:8223-8223(2017).
CC -!- FUNCTION: Bitter taste receptor (PubMed:25796330). Binds quinine,
CC dextromethorphan, diphenhydramine, diphenidol, chlorpheniramine,
CC diphenidol, chloramphenicol, chloroquine and coumarin, this latter
CC being a weak agonist, as well as epiquinidine, ethylhydrocupreine and
CC quinidine (PubMed:28811548). {ECO:0000269|PubMed:25796330,
CC ECO:0000269|PubMed:28811548}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255|RuleBase:RU004424}.
CC -!- TISSUE SPECIFICITY: Expressed in the oral cavity, as well as in the
CC gastrointestinal tract, including in the upper palate, tongue,
CC proventriculus, ventriculus, duodenum, jejunum, ileum, cecum and colon.
CC {ECO:0000269|PubMed:25796330, ECO:0000269|PubMed:26049797}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000255|RuleBase:RU004423}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB249766; BAE80384.1; -; Genomic_DNA.
DR EMBL; AADN04000390; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KT377136; AMA21693.1; -; Genomic_DNA.
DR EMBL; KT377152; AMA21709.1; -; Genomic_DNA.
DR EMBL; KT377138; AMA21695.1; -; Genomic_DNA.
DR EMBL; KT377139; AMA21696.1; -; Genomic_DNA.
DR EMBL; KT377140; AMA21697.1; -; Genomic_DNA.
DR EMBL; KT377145; AMA21702.1; -; Genomic_DNA.
DR EMBL; KT377147; AMA21704.1; -; Genomic_DNA.
DR EMBL; KT377149; AMA21706.1; -; Genomic_DNA.
DR EMBL; KT377151; AMA21708.1; -; Genomic_DNA.
DR RefSeq; XP_004938201.1; XM_004938144.2.
DR AlphaFoldDB; Q2AB83; -.
DR SMR; Q2AB83; -.
DR Ensembl; ENSGALT00000081605; ENSGALP00000051324; ENSGALG00000030506.
DR GeneID; 101749182; -.
DR KEGG; gga:101749182; -.
DR CTD; 259286; -.
DR VEuPathDB; HostDB:geneid_101749182; -.
DR GeneTree; ENSGT00960000186638; -.
DR OMA; FMFLNHC; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; Q2AB83; -.
DR Reactome; R-GGA-418594; G alpha (i) signalling events.
DR Reactome; R-GGA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-GGA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR PRO; PR:Q2AB83; -.
DR Proteomes; UP000000539; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IDA:UniProtKB.
DR GO; GO:1902656; P:calcium ion import into cytosol; IMP:AgBase.
DR GO; GO:0072747; P:cellular response to chloramphenicol; IDA:AgBase.
DR GO; GO:1902350; P:cellular response to chloroquine; IDA:AgBase.
DR GO; GO:1904561; P:cellular response to diphenidol; IDA:AgBase.
DR GO; GO:0071316; P:cellular response to nicotine; IDA:AgBase.
DR GO; GO:0097237; P:cellular response to toxic substance; IDA:AgBase.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IDA:AgBase.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central.
DR GO; GO:1904558; P:response to dextromethorphan; IDA:AgBase.
DR GO; GO:1904560; P:response to diphenidol; IDA:AgBase.
DR GO; GO:0080184; P:response to phenylpropanoid; IDA:AgBase.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..311
FT /note="Taste receptor type 2 member 40"
FT /id="PRO_5010136905"
FT TOPO_DOM 1..9
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..87
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..178
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..266
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MUTAGEN 86
FT /note="K->A: No effect on quinine-, nor diphenhydramine-
FT binding, small decrease in diphenidol- and
FT chlorpheniramine-binding, strong decrease in
FT chloramphenicol- and chloroquine-binding, increase in
FT coumarin-binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 89
FT /note="F->A: Strong increase in quinine-binding, loss of
FT diphenhydramine- and coumarin-binding, small decrease in
FT diphenidol-binding, strong decrease in chlorpheniramine-,
FT chloramphenicol- and chloroquine-binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 93
FT /note="N->A: Loss of quinine-, diphenhydramine- and
FT coumarin-binding, strong decrease in diphenidol-,
FT chlorpheniramine-, chloramphenicol- and chloroquine-
FT binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 181
FT /note="F->A: Loss of quinine-, diphenhydramine- and
FT coumarin-binding, strong decrease in diphenidol-,
FT chloramphenicol- and chloroquine-binding, decrease in
FT chlorpheniramine-binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 185
FT /note="L->A: Strong decrease in quinine- and
FT diphenhydramine-binding, no effect on diphenidol-, nor
FT chlorpheniramine-binding, small decrease in
FT chloramphenicol- and chloroquine-binding, loss of coumarin-
FT binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 244
FT /note="Y->A: Loss of quinine- and diphenhydramine-binding,
FT strong decrease in diphenidol-binding, small decrease in
FT chloramphenicol-binding, increase in coumarin-binding, no
FT effect on chlorpheniramine-, nor chloramphenicol-binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 247
FT /note="N->A: Loss of quinine-, diphenhydramine- and
FT coumarin-binding, strong decrease in diphenidol-binding,
FT small decrease in chlorpheniramine- and chloramphenicol-
FT binding, no effect on chloramphenicol-binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT MUTAGEN 251
FT /note="L->A: Strong decrease in quinine-binding, loss of
FT diphenhydramine-binding, small decrease in diphenidol- and
FT chloramphenicol-binding, no effect on chlorpheniramine-,
FT chloramphenicol-, nor coumarin-binding."
FT /evidence="ECO:0000269|PubMed:28811548"
FT CONFLICT 120
FT /note="S -> T (in Ref. 3; AMA21693/AMA21695/AMA21696/
FT AMA21697/AMA21702/AMA21704/AMA21706/AMA21708/AMA21709)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 311 AA; 35281 MW; FBFFA294F498C093 CRC64;
MSSLFSSFCL VIAIFESVVG LLGNGTIVAV SSTSCIRSKI LSSYDVIVIF LSLSRFFLQL
WMILDFLLIF FCQPSYYEEN LFVTFKTVFI FLNSYSFWFA AWLSVFYCVK VASFTQSFLS
WLKQRIASLI PWMLITSSLF SFATSLPFFW DSYNAHSNFT TPLTMTNSSK RITTRKTNLI
FLILLCNVGI ALPSIMLVFS SILLIRSLWR HTRQMQNNAT GFRDPSLEAL IGAIKTVFSF
LLLYITNFIA LILILSDTFV PLSTEEAICV VVVAACPAGQ SMVLIWSNPR FRELLSSILH
YVNSCVRARC S
