T2R40_HUMAN
ID T2R40_HUMAN Reviewed; 323 AA.
AC P59535; A4D2I2; Q645W6;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Taste receptor type 2 member 40;
DE Short=T2R40;
DE AltName: Full=G-protein coupled receptor 60;
DE AltName: Full=Taste receptor type 2 member 58;
DE Short=T2R58;
GN Name=TAS2R40; Synonyms=GPR60;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12379855; DOI=10.1038/ng1014;
RA Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.;
RT "The human TAS2R16 receptor mediates bitter taste in response to beta-
RT glucopyranosides.";
RL Nat. Genet. 32:397-401(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15496549; DOI=10.1093/molbev/msi027;
RA Fischer A., Gilad Y., Man O., Paeaebo S.;
RT "Evolution of bitter taste receptors in humans and apes.";
RL Mol. Biol. Evol. 22:432-436(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [7]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [8]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Gustducin-coupled receptor implicated in the perception of
CC bitter compounds in the oral cavity and the gastrointestinal tract.
CC Signals through PLCB2 and the calcium-regulated cation channel TRPM5.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and exclusively in gustducin-positive cells.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AF494229; AAM19320.1; -; Genomic_DNA.
DR EMBL; AY724957; AAU21153.1; -; Genomic_DNA.
DR EMBL; CH236959; EAL23782.1; -; Genomic_DNA.
DR EMBL; CH471198; EAW51882.1; -; Genomic_DNA.
DR EMBL; BC101795; AAI01796.1; -; mRNA.
DR EMBL; BC101797; AAI01798.1; -; mRNA.
DR CCDS; CCDS43662.1; -.
DR RefSeq; NP_795363.1; NM_176882.1.
DR AlphaFoldDB; P59535; -.
DR SMR; P59535; -.
DR BioGRID; 129241; 1.
DR STRING; 9606.ENSP00000386210; -.
DR ChEMBL; CHEMBL4523250; -.
DR GlyGen; P59535; 2 sites.
DR iPTMnet; P59535; -.
DR PhosphoSitePlus; P59535; -.
DR BioMuta; TAS2R40; -.
DR DMDM; 29839473; -.
DR PaxDb; P59535; -.
DR PeptideAtlas; P59535; -.
DR PRIDE; P59535; -.
DR Antibodypedia; 32610; 17 antibodies from 11 providers.
DR DNASU; 259286; -.
DR Ensembl; ENST00000408947.4; ENSP00000386210.3; ENSG00000221937.4.
DR GeneID; 259286; -.
DR KEGG; hsa:259286; -.
DR MANE-Select; ENST00000408947.4; ENSP00000386210.3; NM_176882.2; NP_795363.1.
DR UCSC; uc011ksx.3; human.
DR CTD; 259286; -.
DR GeneCards; TAS2R40; -.
DR HGNC; HGNC:18885; TAS2R40.
DR HPA; ENSG00000221937; Tissue enriched (brain).
DR MIM; 613964; gene.
DR neXtProt; NX_P59535; -.
DR PharmGKB; PA38738; -.
DR VEuPathDB; HostDB:ENSG00000221937; -.
DR eggNOG; ENOG502SKRK; Eukaryota.
DR GeneTree; ENSGT00960000186638; -.
DR HOGENOM; CLU_072337_3_0_1; -.
DR InParanoid; P59535; -.
DR OMA; NLWLAAW; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; P59535; -.
DR TreeFam; TF335891; -.
DR PathwayCommons; P59535; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 259286; 11 hits in 1058 CRISPR screens.
DR GeneWiki; TAS2R40; -.
DR GenomeRNAi; 259286; -.
DR Pharos; P59535; Tchem.
DR PRO; PR:P59535; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; P59535; protein.
DR Bgee; ENSG00000221937; Expressed in corpus callosum and 25 other tissues.
DR Genevisible; P59535; HS.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..323
FT /note="Taste receptor type 2 member 40"
FT /id="PRO_0000082288"
FT TOPO_DOM 1..14
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 15..35
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..100
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..162
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..190
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..276
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..323
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 179
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 23
FT /note="V -> L (in dbSNP:rs17164164)"
FT /id="VAR_053350"
FT VARIANT 187
FT /note="S -> Y (in dbSNP:rs10260248)"
FT /id="VAR_053351"
SQ SEQUENCE 323 AA; 36812 MW; 605E66397EE04E77 CRC64;
MATVNTDATD KDISKFKVTF TLVVSGIECI TGILGSGFIT AIYGAEWARG KTLPTGDRIM
LMLSFSRLLL QIWMMLENIF SLLFRIVYNQ NSVYILFKVI TVFLNHSNLW FAAWLKVFYC
LRIANFNHPL FFLMKRKIIV LMPWLLRLSV LVSLSFSFPL SRDVFNVYVN SSIPIPSSNS
TEKKYFSETN MVNLVFFYNM GIFVPLIMFI LAATLLILSL KRHTLHMGSN ATGSRDPSMK
AHIGAIKATS YFLILYIFNA IALFLSTSNI FDTYSSWNIL CKIIMAAYPA GHSVQLILGN
PGLRRAWKRF QHQVPLYLKG QTL
