ID   BPHB_PSEPU              Reviewed;         277 AA.
AC   P72220;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase;
DE            EC=1.3.1.56;
DE   AltName: Full=2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase;
DE   AltName: Full=2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase;
DE   AltName: Full=Biphenyl-2,3-dihydro-2,3-diol dehydrogenase;
DE   AltName: Full=Biphenyl-cis-diol dehydrogenase;
GN   Name=bphB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=OU83;
RX   PubMed=9311131; DOI=10.1111/j.1574-6968.1997.tb12662.x;
RA   Khan A.A., Wang R.F., Nawaz M.S., Cerniglia C.E.;
RT   "Nucleotide sequence of the gene encoding cis-biphenyl dihydrodiol
RT   dehydrogenase (bphB) and the expression of an active recombinant His-tagged
RT   bphB gene product from a PCB degrading bacterium, Pseudomonas putida
RT   OU83.";
RL   FEMS Microbiol. Lett. 154:317-324(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD(+) =
CC         biphenyl-2,3-diol + H(+) + NADH; Xref=Rhea:RHEA:17033,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16205, ChEBI:CHEBI:32922,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.56;
CC   -!- PATHWAY: Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-
CC       pentadienoate and benzoate from biphenyl: step 2/4.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; Y07655; CAA68939.1; -; Genomic_DNA.
DR   AlphaFoldDB; P72220; -.
DR   SMR; P72220; -.
DR   UniPathway; UPA00155; UER00251.
DR   GO; GO:0018509; F:cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017711; BphB_TodD.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR03325; BphB_TodD; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..277
FT                   /note="Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase"
FT                   /id="PRO_0000054535"
FT   ACT_SITE        155
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         9..33
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   277 AA;  28907 MW;  A7048159903BE527 CRC64;
     MKLTGEVVLI TGGASGLGRA LVDRFVAERA KVAVLDKSAE RLAQLETDHG DNVLGVTGDV
     RSLEDQKQAA SRCVAKFGKI DTLIPNAGIW DYSTALIDLP EESLDAAFDE VFHINVKGYI
     HAVKACLPAL VASRGNVIFT ISNAGFYPNG GGPLYTAAKH AVVGLVRELA FELAPYVRVN
     GVGPGGINSD LRGPSSLGMG GKAISTVPLA DMLKSVLPIG RMPEAEEYTG AYVFFATRGD
     AAPATGALLN YDGGLGVRGF FSGAGGNDLL ERLNINS