T2R60_HUMAN
ID T2R60_HUMAN Reviewed; 318 AA.
AC P59551; A4D2G8; Q645W8; Q7RTR7;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Taste receptor type 2 member 60;
DE Short=T2R60;
DE AltName: Full=Taste receptor type 2 member 56;
DE Short=T2R56;
GN Name=TAS2R60;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12584440; DOI=10.1159/000068546;
RA Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.;
RT "Identification and characterization of human taste receptor genes
RT belonging to the TAS2R family.";
RL Cytogenet. Genome Res. 98:45-53(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15496549; DOI=10.1093/molbev/msi027;
RA Fischer A., Gilad Y., Man O., Paeaebo S.;
RT "Evolution of bitter taste receptors in humans and apes.";
RL Mol. Biol. Evol. 22:432-436(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION.
RX PubMed=12679530; DOI=10.1093/molbev/msg083;
RA Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT "Adaptive diversification of bitter taste receptor genes in mammalian
RT evolution.";
RL Mol. Biol. Evol. 20:805-814(2003).
RN [6]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [7]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [8]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC and is gustducin-linked. May play a role in sensing the chemical
CC composition of the gastrointestinal content. The activity of this
CC receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC and lead to the gating of TRPM5 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and exclusively in gustducin-positive cells.
CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC bitter compounds; individual taste cells can discriminate among bitter
CC stimuli.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AY114094; AAM63544.1; -; Genomic_DNA.
DR EMBL; AY724955; AAU21151.1; -; Genomic_DNA.
DR EMBL; CH236959; EAL23790.1; -; Genomic_DNA.
DR EMBL; BC100938; AAI00939.1; -; mRNA.
DR EMBL; BC100939; AAI00940.1; -; mRNA.
DR EMBL; BC100940; AAI00941.1; -; mRNA.
DR EMBL; BK001100; DAA01207.1; -; Genomic_DNA.
DR CCDS; CCDS5885.1; -.
DR RefSeq; NP_803186.1; NM_177437.1.
DR AlphaFoldDB; P59551; -.
DR SMR; P59551; -.
DR BioGRID; 130733; 4.
DR IntAct; P59551; 3.
DR STRING; 9606.ENSP00000327724; -.
DR BindingDB; P59551; -.
DR ChEMBL; CHEMBL3309109; -.
DR GlyGen; P59551; 1 site.
DR PhosphoSitePlus; P59551; -.
DR BioMuta; TAS2R60; -.
DR DMDM; 29839489; -.
DR MassIVE; P59551; -.
DR PaxDb; P59551; -.
DR PeptideAtlas; P59551; -.
DR PRIDE; P59551; -.
DR ProteomicsDB; 57152; -.
DR Antibodypedia; 32663; 37 antibodies from 14 providers.
DR DNASU; 338398; -.
DR Ensembl; ENST00000332690.1; ENSP00000327724.1; ENSG00000185899.1.
DR Ensembl; ENST00000642941.1; ENSP00000494922.1; ENSG00000285341.1.
DR GeneID; 338398; -.
DR KEGG; hsa:338398; -.
DR MANE-Select; ENST00000332690.1; ENSP00000327724.1; NM_177437.1; NP_803186.1.
DR UCSC; uc011ktg.2; human.
DR CTD; 338398; -.
DR DisGeNET; 338398; -.
DR GeneCards; TAS2R60; -.
DR HGNC; HGNC:20639; TAS2R60.
DR HPA; ENSG00000185899; Not detected.
DR MIM; 613968; gene.
DR neXtProt; NX_P59551; -.
DR PharmGKB; PA134879120; -.
DR VEuPathDB; HostDB:ENSG00000185899; -.
DR eggNOG; ENOG502S2SI; Eukaryota.
DR GeneTree; ENSGT00960000186648; -.
DR HOGENOM; CLU_072337_1_1_1; -.
DR InParanoid; P59551; -.
DR OMA; FCLQWVV; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; P59551; -.
DR TreeFam; TF335891; -.
DR PathwayCommons; P59551; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR SignaLink; P59551; -.
DR BioGRID-ORCS; 338398; 12 hits in 1061 CRISPR screens.
DR GeneWiki; TAS2R60; -.
DR GenomeRNAi; 338398; -.
DR Pharos; P59551; Tchem.
DR PRO; PR:P59551; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; P59551; protein.
DR Bgee; ENSG00000185899; Expressed in mucosa of large intestine and 15 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0004930; F:G protein-coupled receptor activity; NAS:UniProtKB.
DR GO; GO:0050913; P:sensory perception of bitter taste; NAS:UniProtKB.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..318
FT /note="Taste receptor type 2 member 60"
FT /id="PRO_0000082351"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..88
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..183
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..234
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..264
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..318
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 179
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 318 AA; 36337 MW; DEDAB1CC8101764B CRC64;
MNGDHMVLGS SVTDKKAIIL VTILLLLRLV AIAGNGFITA ALGVEWVLRR MLLPCDKLLV
SLGASRFCLQ SVVMGKTIYV FLHPMAFPYN PVLQFLAFQW DFLNAATLWS STWLSVFYCV
KIATFTHPVF FWLKHKLSGW LPWMLFSSVG LSSFTTILFF IGNHRMYQNY LRNHLQPWNV
TGDSIRSYCE KFYLFPLKMI TWTMPTAVFF ICMILLITSL GRHRKKALLT TSGFREPSVQ
AHIKALLALL SFAMLFISYF LSLVFSAAGI FPPLDFKFWV WESVIYLCAA VHPIILLFSN
CRLRAVLKSR RSSRCGTP
