ID   T2S1_LACLC              Reviewed;         271 AA.
AC   O34104;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Type II restriction enzyme ScrFI {ECO:0000303|PubMed:12654995};
DE            Short=R.ScrFI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease ScrFI;
DE   AltName: Full=Type-2 restriction enzyme ScrFI;
GN   Name=scrFIR {ECO:0000303|PubMed:9245816};
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=UC503;
RX   PubMed=9245816; DOI=10.1099/00221287-143-7-2277;
RA   Twomey D.P., Gabillet N., Daly C., Fitzgerald G.F.;
RT   "Molecular characterization of the restriction endonuclease gene (scrFIR)
RT   associated with the ScrFI restriction/modification system from Lactococcus
RT   lactis subsp. cremoris UC503.";
RL   Microbiology 143:2277-2286(1997).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CCNGG-3' and cleaves after C-2.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:9245816}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- MISCELLANEOUS: The ScrFI restriction system has two different
CC       methylases. {ECO:0000269|PubMed:9245816}.
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DR   EMBL; U89998; AAB66695.1; -; Genomic_DNA.
DR   RefSeq; WP_015081857.1; NZ_WJUW01000117.1.
DR   AlphaFoldDB; O34104; -.
DR   REBASE; 1639; ScrFI.
DR   PRO; PR:O34104; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019063; Restrct_endonuc_II_LlaMI.
DR   Pfam; PF09562; RE_LlaMI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..271
FT                   /note="Type II restriction enzyme ScrFI"
FT                   /id="PRO_0000077360"
SQ   SEQUENCE   271 AA;  31467 MW;  862D505647DF92BE CRC64;
     MNPNKERLIE IFRSNVKGRI PDISGRNIRH DGRWGHWLEE RFGISANADN HADILGYELK
     NEATSGKTTF GDWSANEYIF KTPPYNSLFS GSTASEKQNA FCRMFGKPNE AKNGRFSWSG
     SPIPKIWQYN SFGQIMVIEE NLDIVIYYSF SQDLRYNKFE IIPPQLQHDE IQIARWYGVA
     NPLLSRRGKT LKDKLEDKFN DLGWFTCTTD SIGAYDKICF GRPITFENWI NLVDSGIVYF
     DSGMYEGNKR PYSQWRADNS YWNSLITDCH Q