ID   T2S1_SALIN              Reviewed;         230 AA.
AC   P09796;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Type II restriction enzyme SinI {ECO:0000303|PubMed:12654995};
DE            Short=R.SinI {ECO:0000303|PubMed:2836359};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease SinI;
DE   AltName: Full=Type-2 restriction enzyme SinI;
GN   Name=sinIR;
OS   Salmonella infantis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=2836359; DOI=10.1128/jb.170.6.2527-2532.1988;
RA   Karreman C., de Waard A.;
RT   "Cloning and complete nucleotide sequences of the type II restriction-
RT   modification genes of Salmonella infantis.";
RL   J. Bacteriol. 170:2527-2532(1988).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGWCC-3' and cleaves after G-1.
CC       {ECO:0000269|PubMed:2836359, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; J03391; AAA27213.1; -; Genomic_DNA.
DR   RefSeq; WP_023214691.1; NZ_WACL01000006.1.
DR   AlphaFoldDB; P09796; -.
DR   SMR; P09796; -.
DR   REBASE; 1687; SinI.
DR   PATRIC; fig|595.18.peg.4607; -.
DR   PRO; PR:P09796; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019070; Restrct_endonuc_II_SinI.
DR   Pfam; PF09570; RE_SinI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..230
FT                   /note="Type II restriction enzyme SinI"
FT                   /id="PRO_0000077361"
SQ   SEQUENCE   230 AA;  26870 MW;  95BAD4E421E72CAF CRC64;
     MSEAVFFVEN AEELAKQKMD NINPELSEKF QLLIKFLSRF PESCSNPRSK QVRKNFGKAE
     HIEYLAQNFN ESRLPKKPTP PTTIPDEVVS LVLNVSFDIP QENLNRIKEE HRLSMASENI
     VGDLLERYLA EKLEPCGWIW CSGTSVKAVD FIHYDNEKDE WGLLQVKNRD NTENSSSSKI
     RDNTPIKKWF RTFSQRDATN WENFPDEVSS KDLNEDDFRA FVESYLRKIK