ID   T2S1_STRAH              Reviewed;         358 AA.
AC   O31074;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Type II restriction enzyme SacI {ECO:0000303|PubMed:12654995};
DE            Short=R.SacI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease SacI;
DE   AltName: Full=Type-2 restriction enzyme SacI;
GN   Name=sacIR {ECO:0000303|PubMed:9862476};
OS   Streptomyces achromogenes.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=67255;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL
RC   B-2120;
RX   PubMed=9862476; DOI=10.1007/s004380050890;
RA   Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L.,
RA   Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J.,
RA   Slatko B., Benner J. II;
RT   "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI
RT   restriction-modification systems in Escherichia coli.";
RL   Mol. Gen. Genet. 260:226-231(1998).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A subtype P restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GAGCTC-3' and cleaves after T-5.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:9862476}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; AF027867; AAC97119.1; -; Genomic_DNA.
DR   AlphaFoldDB; O31074; -.
DR   BRENDA; 3.1.21.4; 5915.
DR   PRO; PR:O31074; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019066; Restrct_endonuc_II_SacI.
DR   Pfam; PF09566; RE_SacI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..358
FT                   /note="Type II restriction enzyme SacI"
FT                   /id="PRO_0000077364"
SQ   SEQUENCE   358 AA;  39965 MW;  EBF68CB8037EBF13 CRC64;
     MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA LVTALLARAC
     DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG ATGREPINNQ PFFRYDQYSE
     IVRVQTKARP YLDRVSSALA RVDEEDYSTE ESFRALVAVL AVCISVANKK QRVAVGSAIV
     EASLIAETQS FVVSGHDVPR KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD
     PLLTVEVRGK SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC
     IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR WAEIFPET