ID   T2S1_STRAL              Reviewed;         315 AA.
AC   Q53608;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Type II restriction enzyme SalI {ECO:0000303|PubMed:12654995};
DE            Short=R.SalI {ECO:0000303|PubMed:7828868};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease SalI;
DE   AltName: Full=Type-2 restriction enzyme SalI;
GN   Name=salIR {ECO:0000303|PubMed:7828868};
OS   Streptomyces albus G.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7828868; DOI=10.1016/0378-1119(94)90650-5;
RA   Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G.;
RT   "Organization and sequence of the SalI restriction-modification system.";
RL   Gene 151:167-172(1994).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GTCGAC-3' and cleaves after G-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; U01232; AAA81886.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q53608; -.
DR   REBASE; 1588; SalI.
DR   PRO; PR:Q53608; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019072; Restrct_endonuc_II_XamI.
DR   Pfam; PF09572; RE_XamI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..315
FT                   /note="Type II restriction enzyme SalI"
FT                   /id="PRO_0000077365"
SQ   SEQUENCE   315 AA;  35337 MW;  DA7C3CA88E924F07 CRC64;
     MINADKPHRW NDDVQASVRL YNQWFLDAAP KAYRDTRQLT IDEVEQAFQR TANMTSITPE
     VLKAHPKTLA TLRMSTAPPI ARDRLVGLSH GSKSLLDTME KGKLPPRMKG DVLDTHLAKM
     CAVLTDLLDL DLFHWYPTGE PAEPRQRELA ATVVADRLCG AIADPIVRNA QERRQLALIE
     EWLLARGYTK KTHSASLPLN TMQPGTFSFR QNVVVGSDLP VNIPVDAVIQ PHTPHSHKLP
     ILIEAKSAGD FTNTNKRRKE EATKIHQLQL KYGNEISLTL FLCGYFNTGY LGYSAAEGLD
     WVWEHRIDDL EAAGA