T2S1_STRFI
ID T2S1_STRFI Reviewed; 269 AA.
AC O52512;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Type II restriction enzyme SfiI {ECO:0000303|PubMed:12654995};
DE Short=R.SfiI;
DE EC=3.1.21.4;
DE AltName: Full=Endonuclease SfiI;
DE AltName: Full=Type-2 restriction enzyme SfiI;
GN Name=sfiIR;
OS Streptomyces fimbriatus.
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=68197;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G.;
RT "Characterization of the SfiI restriction and modification genes.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE, AND SUBTYPES.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: An F and P subtype restriction enzyme that recognizes the
CC double-stranded sequence 5'-GGCCN(5)GGCC-3' and cleaves before N-9.
CC {ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR EMBL; AF039750; AAB95365.1; -; Genomic_DNA.
DR PDB; 2EZV; X-ray; 2.40 A; A/B=1-269.
DR PDB; 2F03; X-ray; 3.05 A; A/C=1-269.
DR PDBsum; 2EZV; -.
DR PDBsum; 2F03; -.
DR AlphaFoldDB; O52512; -.
DR SMR; O52512; -.
DR REBASE; 1655; SfiI.
DR EvolutionaryTrace; O52512; -.
DR PRO; PR:O52512; -.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 2.40.50.610; -; 1.
DR Gene3D; 3.40.600.40; -; 1.
DR InterPro; IPR021580; Restrct_endonuc_II_SfiI.
DR InterPro; IPR043118; Restrct_endonuc_II_SfiI_dom1.
DR InterPro; IPR043117; Restrct_endonuc_II_SfiI_dom2.
DR Pfam; PF11487; RestrictionSfiI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endonuclease; Hydrolase; Nuclease; Restriction system.
FT CHAIN 1..269
FT /note="Type II restriction enzyme SfiI"
FT /id="PRO_0000077366"
FT HELIX 5..7
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 10..41
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 49..62
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:2F03"
FT STRAND 79..86
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 93..105
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 114..116
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 121..124
FT /evidence="ECO:0007829|PDB:2EZV"
FT TURN 126..128
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 130..134
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 140..145
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 147..167
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 175..186
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 189..191
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 192..195
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 203..206
FT /evidence="ECO:0007829|PDB:2EZV"
FT TURN 211..214
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 219..221
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 223..229
FT /evidence="ECO:0007829|PDB:2EZV"
FT HELIX 231..233
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 234..238
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 244..246
FT /evidence="ECO:0007829|PDB:2F03"
FT STRAND 249..253
FT /evidence="ECO:0007829|PDB:2EZV"
FT STRAND 259..263
FT /evidence="ECO:0007829|PDB:2EZV"
SQ SEQUENCE 269 AA; 31044 MW; 3C48499BAA5205EA CRC64;
MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV IVLAEDITQY
ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD AKASTEKNRD TLQRSQLPMD
AEFRNTSSGE VVTMEAGVIP HLMLQSANDG VLPAVTTSIF VHFYYRELKD VEGRYRELKS
IYVLSLPHAR LKQRYNPDPD TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS
ADSEYTQPRW RDLNDAGHEV TKEFLFLER
