ID   T2S2_SHISO              Reviewed;         305 AA.
AC   P34880;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Type II restriction enzyme SsoII {ECO:0000303|PubMed:12654995};
DE            Short=R.SsoII {ECO:0000303|PubMed:7916706};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease SsoII;
DE   AltName: Full=Type-2 restriction enzyme SsoII;
GN   Name=ssoIIR;
OS   Shigella sonnei.
OG   Plasmid P4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=624;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=47;
RX   PubMed=7916706; DOI=10.1016/0378-1119(93)90756-s;
RA   Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.;
RT   "Analysis of the nucleotide and derived amino acid sequences of the SsoII
RT   restriction endonuclease and methyltransferase.";
RL   Gene 124:13-19(1993).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CCNGG-3' and cleaves before C-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; M86545; AAA98280.1; -; Genomic_DNA.
DR   PIR; JU0156; JU0156.
DR   RefSeq; WP_001189381.1; NZ_UEDG01000221.1.
DR   AlphaFoldDB; P34880; -.
DR   SMR; P34880; -.
DR   REBASE; 1739; SsoII.
DR   PRO; PR:P34880; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.91.80; -; 1.
DR   InterPro; IPR038365; EcoRII_C_sf.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR015109; Restrct_endonuc_II_EcoRII_C.
DR   Pfam; PF09019; EcoRII-C; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Restriction system.
FT   CHAIN           1..305
FT                   /note="Type II restriction enzyme SsoII"
FT                   /id="PRO_0000077363"
SQ   SEQUENCE   305 AA;  35939 MW;  61FDF54AB0C02D29 CRC64;
     MQRLSPGEFK TLISKERKSH FITPFALVYK TFCDLGYDQK NSDYFLNNPS EYIIAMRKNC
     WKEFEPFEKE FTTRMLSYLI DEERIKDMSP YDAIRDFTME YPTHIYDLAL SNTQSRRSRA
     GKEFESILEL LMMGAGIPVD VQGAIGKSFF QKNQIGKLVD LVMPGVVQYT SNKRNTMLIS
     AKTTLRERWQ EVPEEVNRTG IREMYLATLD DSFSEETINI LYEANVVVVT TIENKNFKYK
     NNNRVLTFED MLQSAMELSR KWNNVSYTDS EKEEIQRSIL KQIEKYSDFP YVVNYYRNRL
     SALFD