ID   T2T8_THET8              Reviewed;         263 AA.
AC   P29748; Q5SHZ6;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Type II restriction enzyme TthHB8I {ECO:0000303|PubMed:12654995};
DE            Short=R.TthHB8I;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease TthHB8I {ECO:0000303|PubMed:1339363};
DE   AltName: Full=Type-2 restriction enzyme TthHB8I;
GN   Name=tthHB8IR; OrderedLocusNames=TTHA1584;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1339363; DOI=10.1016/0378-1119(92)90296-2;
RA   Barany F., Danzitz M., Zebala J., Mayer A.;
RT   "Cloning and sequencing of genes encoding the TthHB8I restriction and
RT   modification enzymes: comparison with the isoschizomeric TaqI enzymes.";
RL   Gene 112:3-12(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-TCGA-3' and cleaves after T-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; M76680; AAA27490.1; -; Genomic_DNA.
DR   EMBL; M74795; AAA27489.1; -; Genomic_DNA.
DR   EMBL; AP008226; BAD71407.1; -; Genomic_DNA.
DR   RefSeq; WP_011228787.1; NC_006461.1.
DR   RefSeq; YP_144850.1; NC_006461.1.
DR   AlphaFoldDB; P29748; -.
DR   STRING; 300852.55772966; -.
DR   REBASE; 1831; TthHB8I.
DR   PRIDE; P29748; -.
DR   EnsemblBacteria; BAD71407; BAD71407; BAD71407.
DR   GeneID; 3170138; -.
DR   KEGG; ttj:TTHA1584; -.
DR   eggNOG; ENOG5032U4T; Bacteria.
DR   HOGENOM; CLU_1072404_0_0_0; -.
DR   OMA; ARMYRTW; -.
DR   PRO; PR:P29748; -.
DR   Proteomes; UP000000532; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019073; Restrct_endonuc_II_TaqI.
DR   Pfam; PF09573; RE_TaqI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Restriction system.
FT   CHAIN           1..263
FT                   /note="Type II restriction enzyme TthHB8I"
FT                   /id="PRO_0000077370"
FT   CONFLICT        119
FT                   /note="C -> S (in Ref. 1; AAA27490/AAA27489)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   263 AA;  31584 MW;  10EF1995DB291D98 CRC64;
     MAPTQAQKVL EAFEDFLKCL DLESYQEKYR PIKTVEQDLP RELNPLPDLY DHYWKPNGNT
     LHFPDFETFF DQWWEKRLRP LNEFIRKYFW GCSYEFVRLG LEARLYRTAV SIWTQFHFCY
     RWNASCQLRL TATWELDAQG IDAQIQAEDR LIGIQIKKET YRSEAREGNR FLRRREHSAL
     LEVPYTLQSP EELERKAQRA RTREEAYRLW VKIAHHLERL PNGFVIFRES YVKDLENFLK
     QNATTLSGLI PWDKVAREAL TGP