ID   T2V1_GEOSE              Reviewed;         224 AA.
AC   P43421;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Type II restriction enzyme BstVI {ECO:0000303|PubMed:12654995};
DE            Short=R.BstVI {ECO:0000303|PubMed:8370531};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease BstVI;
DE   AltName: Full=Type-2 restriction enzyme BstVI;
GN   Name=bstVIR {ECO:0000303|PubMed:8370531};
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=V;
RX   PubMed=8370531; DOI=10.1016/0378-1119(93)90676-t;
RA   Gonzalez E., Vasquez C.;
RT   "Characterization of the bstVIRM genes encoding the Bacillus
RT   stearothermophilus V restriction-modification system.";
RL   Gene 131:103-106(1993).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CTCGAG-3' and cleaves after C-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- SIMILARITY: Belongs to the XhoI type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; L07643; AAA22281.1; -; Genomic_DNA.
DR   PIR; JN0798; JN0798.
DR   AlphaFoldDB; P43421; -.
DR   REBASE; 594; BstVI.
DR   PRO; PR:P43421; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR007636; Restrct_endonuc_II_XhoI.
DR   Pfam; PF04555; XhoI; 1.
DR   PIRSF; PIRSF000994; Restrict_endonuc_II_XhoI; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..224
FT                   /note="Type II restriction enzyme BstVI"
FT                   /id="PRO_0000077289"
SQ   SEQUENCE   224 AA;  25155 MW;  FE6767D6DBC72F1A CRC64;
     MNKQASGKDT GNRGAVTGGK QMDGFADMLT NLLSKVGISN HEVFTSEKPH LPGYFRATKK
     WDFLVVKTND RGEKYLLAAV ELKSHVGPSF GNNLNNRVEE SLGSATDIWI AFREKAFKNS
     RAPWLGYLML LEDCPQSTKP VKVDEPHFEV FPEFKNASYA KRYELFCRKI VLERKYTSSC
     LLMSDKQGGL QGIYKEPAND LKIYPFLYSL LTHVATEAAL ISSS