ID   T2X1_XANCR              Reviewed;         274 AA.
AC   P96189;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Type II restriction enzyme XamI {ECO:0000303|PubMed:12654995};
DE            Short=R.XamI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease XamI;
DE   AltName: Full=Type-2 restriction enzyme XamI;
GN   Name=xamIR;
OS   Xanthomonas campestris pv. amaranthicola.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=54735;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11645 / LMG 498 / NCPPB 570;
RX   PubMed=9130589; DOI=10.1016/s0167-4781(97)00030-4;
RA   Gomez P., Ribas-Aparicio R.M., Pelaez A.I., Gomez A., Rodicio M.R.;
RT   "Isolation and nucleotide sequence of the gene encoding the XamI DNA
RT   methyltransferase of Xanthomonas campestris pv. amaranthicola.";
RL   Biochim. Biophys. Acta 1351:261-266(1997).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GTCGAC-3' and cleaves after G-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; U77781; AAD13687.1; -; Genomic_DNA.
DR   AlphaFoldDB; P96189; -.
DR   REBASE; 2125; XamI.
DR   PRO; PR:P96189; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019072; Restrct_endonuc_II_XamI.
DR   Pfam; PF09572; RE_XamI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..274
FT                   /note="Type II restriction enzyme XamI"
FT                   /id="PRO_0000077373"
SQ   SEQUENCE   274 AA;  30170 MW;  F7617A101DC198AC CRC64;
     MTSAPPRWTT AELAEDAATS AAQFRTERLA VTDSWGTHYN QARGKFELLF KKLSDLNPEA
     ITDDNLAEAY GLGLGEALRY LAGPPISDDD LQVIADVESI APGVLKKNAE ALRKVFGVIE
     RVIDPHRFPW METGGMPTEQ QREAALLASS VLLAAQRIAT ERRNEGKDNQ ETMVKDYLRT
     LGFTEAPAAA INTIVKGPQA MQFCAECQLG ERKADVVVRL HDTRLMAIEC KVSNSSTNSV
     KRLNNDAAVK AEYWIKQFGT AQVVPSRSLV SSRY