ID   T2X1_XANVA              Reviewed;         248 AA.
AC   Q9KVZ7;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Type II restriction enzyme XhoI {ECO:0000303|PubMed:12654995};
DE            Short=R.XhoI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease XhoI;
DE   AltName: Full=Type-2 restriction enzyme XhoI;
OS   Xanthomonas vasicola.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=56459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13461 / XH 3 / LMG 7416;
RA   Morita M., Sugino Y.;
RT   "Nucleotide sequences of the XhoI methylase and endonuclease genes.";
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CTCGAG-3' and cleaves after C-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- SIMILARITY: Belongs to the XhoI type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; AB046567; BAB03597.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9KVZ7; -.
DR   ChEMBL; CHEMBL2439945; -.
DR   BRENDA; 3.1.21.4; 6712.
DR   PRO; PR:Q9KVZ7; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR007636; Restrct_endonuc_II_XhoI.
DR   Pfam; PF04555; XhoI; 1.
DR   PIRSF; PIRSF000994; Restrict_endonuc_II_XhoI; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..248
FT                   /note="Type II restriction enzyme XhoI"
FT                   /id="PRO_0000077372"
SQ   SEQUENCE   248 AA;  27575 MW;  81D3B2B231BE6622 CRC64;
     MALDLAEYDR LARLGVAQFW DGRSSALEND EERSQGGERS GVLGGRNMDG FLAMIEGIVR
     KNGLPDAEVC IKGRPNLTLP GYYRPTKLWD VLVFDGKKLV AAVELKSHVG PSFGNNFNNR
     AEEAIGTAHD LATAIREGIL GDQLPPFTGW LILVEDCEKS KRAVRDSSPH FPVFPDFKGA
     SYLTRYEVLC RKLILKGFTP RPQSLLRPAL RVLGATIASF RKPRVCAHLR HGWPAMYPVG
     QSRIRVNF