T30A1_MOUSE
ID T30A1_MOUSE Reviewed; 664 AA.
AC Q99J38; Q8C0X1;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Tetratricopeptide repeat protein 30A1;
DE Short=TPR repeat protein 30A1;
GN Name=Ttc30a1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NMRI; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH IFT52.
RX PubMed=23810713; DOI=10.1016/j.yexcr.2013.06.010;
RA Howard P.W., Jue S.F., Maurer R.A.;
RT "Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport
RT complex B proteins and KIF17.";
RL Exp. Cell Res. 319:2275-2281(2013).
CC -!- FUNCTION: Required for polyglutamylation of axonemal tubulin. Plays a
CC role in anterograde intraflagellar transport (IFT), the process by
CC which cilia precursors are transported from the base of the cilium to
CC the site of their incorporation at the tip. {ECO:0000250}.
CC -!- SUBUNIT: Interacts wit the IFT B complex component IFT52.
CC {ECO:0000269|PubMed:23810713}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TTC30/dfy-1/fleer family. {ECO:0000305}.
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DR EMBL; AK029535; BAC26502.1; -; mRNA.
DR EMBL; AK045096; BAC32220.1; -; mRNA.
DR EMBL; AL772341; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC004773; AAH04773.1; -; mRNA.
DR CCDS; CCDS16154.1; -.
DR RefSeq; NP_084464.3; NM_030188.3.
DR AlphaFoldDB; Q99J38; -.
DR SMR; Q99J38; -.
DR STRING; 10090.ENSMUSP00000097574; -.
DR iPTMnet; Q99J38; -.
DR PhosphoSitePlus; Q99J38; -.
DR EPD; Q99J38; -.
DR jPOST; Q99J38; -.
DR MaxQB; Q99J38; -.
DR PaxDb; Q99J38; -.
DR PRIDE; Q99J38; -.
DR ProteomicsDB; 254638; -.
DR DNASU; 78802; -.
DR Ensembl; ENSMUST00000099994; ENSMUSP00000097574; ENSMUSG00000075271.
DR GeneID; 78802; -.
DR KEGG; mmu:78802; -.
DR UCSC; uc008kew.3; mouse.
DR CTD; 78802; -.
DR MGI; MGI:1926052; Ttc30a1.
DR VEuPathDB; HostDB:ENSMUSG00000075271; -.
DR eggNOG; KOG4340; Eukaryota.
DR GeneTree; ENSGT00390000010116; -.
DR HOGENOM; CLU_023760_0_0_1; -.
DR InParanoid; Q99J38; -.
DR OMA; KHTIMLS; -.
DR OrthoDB; 247838at2759; -.
DR PhylomeDB; Q99J38; -.
DR TreeFam; TF314592; -.
DR BioGRID-ORCS; 78802; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Ttc30a1; mouse.
DR PRO; PR:Q99J38; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q99J38; protein.
DR Bgee; ENSMUSG00000075271; Expressed in spermatocyte and 213 other tissues.
DR Genevisible; Q99J38; MM.
DR GO; GO:0005879; C:axonemal microtubule; IBA:GO_Central.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0030992; C:intraciliary transport particle B; IDA:MGI.
DR GO; GO:0120170; F:intraciliary transport particle B binding; IDA:MGI.
DR GO; GO:0035720; P:intraciliary anterograde transport; IC:MGI.
DR GO; GO:0042073; P:intraciliary transport; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR039941; TT30.
DR PANTHER; PTHR20931; PTHR20931; 1.
DR Pfam; PF13176; TPR_7; 1.
DR SMART; SM00028; TPR; 4.
DR SUPFAM; SSF48452; SSF48452; 3.
DR PROSITE; PS50293; TPR_REGION; 3.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..664
FT /note="Tetratricopeptide repeat protein 30A1"
FT /id="PRO_0000333202"
FT REPEAT 11..44
FT /note="TPR 1"
FT REPEAT 45..78
FT /note="TPR 2"
FT REPEAT 153..186
FT /note="TPR 3"
FT REPEAT 188..220
FT /note="TPR 4"
FT REPEAT 393..423
FT /note="TPR 5"
FT REPEAT 424..456
FT /note="TPR 6"
FT REPEAT 458..491
FT /note="TPR 7"
FT REPEAT 543..576
FT /note="TPR 8"
FT COILED 507..534
FT /evidence="ECO:0000255"
FT CONFLICT 2
FT /note="A -> G (in Ref. 1; BAC26502)"
FT /evidence="ECO:0000305"
FT CONFLICT 561
FT /note="F -> L (in Ref. 1; BAC26502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 664 AA; 76238 MW; F9C3C7863231CEA2 CRC64;
MAWQSSSKVP DGEFTAVVYR LIRDSRYSEA VQLLSAELQR SSRSRAGLSL LAYCYYRLQE
FELAAECYEQ LSQMHPELEQ YRLYQAQALY KACLYPEATR VTFLLDNPAY QTRVLRLQAA
IKYSEGDLPG ARSLVEQLLS GEAGEDSGGE NDPDGLVNMG CLLYKEGHYE AACSKFLAAL
QASGYQPDLS YNLALAYYSS RQYAPALKHI ADIIERGIRQ HPELGVGMTT EGIDVRSVGN
TVVLHQTALI EAFNLKAAIE YQLRNFEVAQ ETLTDMPPRA EEELDPVTLH NQALMNMDAK
PTEGFEKLQF LLQQNPFPPE TFGNLLLLYC KYEYFDLAAD VLAENAHLTY KFLTPYLYDF
LDAMITCQTA PEEAFIKLDG LAGMLTEQLR RLTKQVQEAR HNRDDEIIKK AMNEYDETLE
KYIPVLMAQA KIYWNLENYP MVEKIFRKSV EFCNDHDVWK LNVAHVLFMQ ENKYKEAIGF
YEPIVKKNYD NILSVSAIVL ANLCVSYIMT SQNEEAEELM RKIEKEEEQL SYGDPDKKIY
HLCIVNLVIG TLYCAKGNYD FGISRVIKSL EPYHKKLGTD TWYYAKRCFL SLLENMSKHM
IVLCDGVVQE CVQFLEYCEL YGRNIPAVLE QPLEEERIHT GKNTVTYESR LLKALIYEVI
GWNM
