ID   T3C3_HOLCU              Reviewed;          38 AA.
AC   P15968;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Mu-agatoxin-Hc1c;
DE            Short=Mu-AGTX-Hc1c;
DE   AltName: Full=Curtatoxin-3 {ECO:0000303|PubMed:2298738};
DE            Short=CT-III {ECO:0000303|PubMed:2298738};
OS   Hololena curta (Funnel-web spider) (Agelena curta).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Hololena.
OX   NCBI_TaxID=6910;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AMIDATION AT SER-38, AND
RP   TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=2298738; DOI=10.1016/s0021-9258(19)39939-9;
RA   Stapleton A., Blankenship D.T., Ackermann D.L., Chen T.-M., Gorder G.W.,
RA   Manley G.D., Palfreyman M.G., Coutant J.E., Cardin A.D.;
RT   "Curtatoxins. Neurotoxic insecticidal polypeptides isolated from the
RT   funnel-web spider Hololena curta.";
RL   J. Biol. Chem. 265:2054-2059(1990).
RN   [2]
RP   REVIEW.
RX   PubMed=15066410; DOI=10.1016/j.toxicon.2004.02.004;
RA   Adams M.E.;
RT   "Agatoxins: ion channel specific toxins from the American funnel web
RT   spider, Agelenopsis aperta.";
RL   Toxicon 43:509-525(2004).
CC   -!- FUNCTION: Insecticidal neurotoxin that induces irreversible
CC       neuromuscular blockade in house crickets (A.domesticus). Modifies
CC       presynaptic voltage-gated sodium channels (Nav), causing them to open
CC       at the normal resting potential of the nerve. This leads to spontaneous
CC       release of neurotransmitter and repetitive action potentials in motor
CC       neurons. {ECO:0000269|PubMed:2298738}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2298738}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:2298738}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- TOXIC DOSE: LD(50) is 4 mg/kg in house crickets (Acheta domesticus).
CC       {ECO:0000269|PubMed:2298738}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 07 (Beta/delta-agtx) family. 02
CC       (aga-3) subfamily. {ECO:0000305}.
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DR   PIR; C35030; C35030.
DR   AlphaFoldDB; P15968; -.
DR   SMR; P15968; -.
DR   ArachnoServer; AS000296; mu-agatoxin-Hc1c.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR016328; Beta/delta-agatoxin_fam.
DR   PIRSF; PIRSF001882; Curtatoxin; 1.
DR   PROSITE; PS60015; MU_AGATOXIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   PEPTIDE         1..38
FT                   /note="Mu-agatoxin-Hc1c"
FT                   /id="PRO_0000044957"
FT   MOD_RES         38
FT                   /note="Serine amide"
FT                   /evidence="ECO:0000269|PubMed:2298738"
FT   DISULFID        3..19
FT                   /evidence="ECO:0000250"
FT   DISULFID        10..24
FT                   /evidence="ECO:0000250"
FT   DISULFID        18..34
FT                   /evidence="ECO:0000250"
FT   DISULFID        26..32
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   38 AA;  4245 MW;  6741A8F4DB20FB59 CRC64;
     ADCVGDGQKC ADWFGPYCCS GYYCSCRSMP YCRCRSDS