T3HPD_AGRVS
ID T3HPD_AGRVS Reviewed; 342 AA.
AC B9K4G4;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Trans-3-hydroxy-L-proline dehydratase {ECO:0000303|PubMed:24980702};
DE Short=T3LHyp dehydratase;
DE Short=t3HypD {ECO:0000303|PubMed:24980702};
DE EC=4.2.1.77 {ECO:0000269|PubMed:24980702};
DE AltName: Full=Trans-L-3-hydroxyproline dehydratase;
GN OrderedLocusNames=Avi_7022 {ECO:0000312|EMBL:ACM39762.1};
OS Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain
OS S4)).
OG Plasmid pAtS4e {ECO:0000312|EMBL:ACM39762.1,
OG ECO:0000312|Proteomes:UP000001596}.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX NCBI_TaxID=311402;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S4 / ATCC BAA-846;
RX PubMed=19251847; DOI=10.1128/jb.01779-08;
RA Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K.,
RA Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L.,
RA Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A.,
RA Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M.,
RA Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N.,
RA Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W.;
RT "Genome sequences of three Agrobacterium biovars help elucidate the
RT evolution of multichromosome genomes in bacteria.";
RL J. Bacteriol. 191:2501-2511(2009).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH PYRROLE
RP 2-CARBOXYLATE, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES,
RP AND ACTIVE SITE.
RC STRAIN=S4 / ATCC BAA-846;
RX PubMed=24980702; DOI=10.7554/elife.03275;
RA Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B.,
RA San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A.,
RA Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.;
RT "Prediction and characterization of enzymatic activities guided by sequence
RT similarity and genome neighborhood networks.";
RL Elife 3:E03275-E03275(2014).
CC -!- FUNCTION: Catalyzes the dehydration of trans-3-hydroxy-L-proline
CC (t3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C). Is likely
CC involved in a degradation pathway that converts t3LHyp to L-proline.
CC Displays neither proline racemase activity nor 4-hydroxyproline 2-
CC epimerase activity. {ECO:0000269|PubMed:24980702, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=trans-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate + H2O;
CC Xref=Rhea:RHEA:10320, ChEBI:CHEBI:15377, ChEBI:CHEBI:39785,
CC ChEBI:CHEBI:57938; EC=4.2.1.77;
CC Evidence={ECO:0000269|PubMed:24980702};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=15 mM for trans-3-hydroxy-L-proline {ECO:0000269|PubMed:24980702};
CC Note=kcat is 4.3 sec(-1) for t3LHyp dehydration.
CC {ECO:0000269|PubMed:24980702};
CC -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
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DR EMBL; CP000638; ACM39762.1; -; Genomic_DNA.
DR RefSeq; WP_015918209.1; NC_011981.1.
DR PDB; 4K7G; X-ray; 2.00 A; B/D=1-342.
DR PDBsum; 4K7G; -.
DR AlphaFoldDB; B9K4G4; -.
DR SMR; B9K4G4; -.
DR STRING; 311402.Avi_7022; -.
DR EnsemblBacteria; ACM39762; ACM39762; Avi_7022.
DR KEGG; avi:Avi_7022; -.
DR eggNOG; COG3938; Bacteria.
DR HOGENOM; CLU_036729_2_0_5; -.
DR OMA; IMESEEY; -.
DR OrthoDB; 559014at2; -.
DR SABIO-RK; B9K4G4; -.
DR Proteomes; UP000001596; Plasmid pAtS4e.
DR GO; GO:0050346; F:trans-L-3-hydroxyproline dehydratase activity; IDA:CACAO.
DR InterPro; IPR008794; Pro_racemase_fam.
DR PANTHER; PTHR33442; PTHR33442; 1.
DR Pfam; PF05544; Pro_racemase; 1.
DR PIRSF; PIRSF029792; Pro_racemase; 1.
DR SFLD; SFLDS00028; Proline_Racemase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; Plasmid; Reference proteome.
FT CHAIN 1..342
FT /note="Trans-3-hydroxy-L-proline dehydratase"
FT /id="PRO_0000432250"
FT ACT_SITE 90
FT /note="Proton acceptor"
FT /evidence="ECO:0000305|PubMed:24980702"
FT BINDING 91..92
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:24980702"
FT BINDING 252
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:24980702"
FT BINDING 257..258
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:24980702"
FT STRAND 7..14
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 20..25
FT /evidence="ECO:0007829|PDB:4K7G"
FT HELIX 34..44
FT /evidence="ECO:0007829|PDB:4K7G"
FT HELIX 46..52
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 63..67
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:4K7G"
FT HELIX 91..103
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 111..120
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 123..132
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 135..142
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 146..157
FT /evidence="ECO:0007829|PDB:4K7G"
FT TURN 158..160
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 161..178
FT /evidence="ECO:0007829|PDB:4K7G"
FT TURN 180..183
FT /evidence="ECO:0007829|PDB:4K7G"
FT HELIX 188..190
FT /evidence="ECO:0007829|PDB:4K7G"
FT HELIX 191..208
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 224..228
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 232..234
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 237..246
FT /evidence="ECO:0007829|PDB:4K7G"
FT TURN 247..249
FT /evidence="ECO:0007829|PDB:4K7G"
FT HELIX 257..269
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 279..282
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 288..299
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 302..311
FT /evidence="ECO:0007829|PDB:4K7G"
FT STRAND 313..321
FT /evidence="ECO:0007829|PDB:4K7G"
FT TURN 336..338
FT /evidence="ECO:0007829|PDB:4K7G"
SQ SEQUENCE 342 AA; 36363 MW; 67E15D7878ABF529 CRC64;
MRTNKVIHVI GVHAEGEVGD VIVGGVSPPP GDTLWEQSRF IASDETLRNF VLNEPRGGVF
RHVNLLVPPK DPRAQMGFII MEPADTPPMS GSNSICVSTA ILDSGIISMQ EPLTHMVLEA
PGGVIEVTAE CANGKAERIN VLNVASFVTR LAAALEVEGL GTLTVDTAYG GDSFVIVDAI
GLGFSLKPDE ARELAELGMK ITAAANEQLG FVHPCNADWN HISFCQMTTP ITRENGILTG
KSAVAIRPGK IDRSPTGTGC SARLAVMHAR GEIGIGETYI GRSIIDSEFK CHIDSLTEIG
GLSAIRPVIS GRAWITGVSQ LMLDPTDPWP SGYQLSDTWP AI
