ID   T3HPD_STAND             Reviewed;         345 AA.
AC   D7A0Y1;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Trans-3-hydroxy-L-proline dehydratase {ECO:0000303|PubMed:25608448};
DE            Short=T3LHyp dehydratase {ECO:0000303|PubMed:25608448};
DE            Short=t3HypD {ECO:0000303|PubMed:25608448};
DE            EC=4.2.1.77 {ECO:0000269|PubMed:25608448};
GN   OrderedLocusNames=Snov_0155 {ECO:0000312|EMBL:ADH87491.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM
OS   12100 / NBRC 12443 / NCIMB 10456).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443
RC   / NCIMB 10456;
RX   PubMed=23450099; DOI=10.4056/sigs.3006378;
RA   Kappler U., Davenport K., Beatson S., Lucas S., Lapidus A., Copeland A.,
RA   Berry K.W., Glavina Del Rio T., Hammon N., Dalin E., Tice H., Pitluck S.,
RA   Richardson P., Bruce D., Goodwin L.A., Han C., Tapia R., Detter J.C.,
RA   Chang Y.J., Jeffries C.D., Land M., Hauser L., Kyrpides N.C., Goker M.,
RA   Ivanova N., Klenk H.P., Woyke T.;
RT   "Complete genome sequence of the facultatively chemolithoautotrophic and
RT   methylotrophic alpha Proteobacterium Starkeya novella type strain (ATCC
RT   8093(T)).";
RL   Stand. Genomic Sci. 7:44-58(2012).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443
RC   / NCIMB 10456;
RX   PubMed=25608448; DOI=10.1021/ja5103986;
RA   Zhang X., Kumar R., Vetting M.W., Zhao S., Jacobson M.P., Almo S.C.,
RA   Gerlt J.A.;
RT   "A unique cis-3-hydroxy-L-proline dehydratase in the enolase superfamily.";
RL   J. Am. Chem. Soc. 137:1388-1391(2015).
CC   -!- FUNCTION: Catalyzes the dehydration of trans-3-hydroxy-L-proline
CC       (t3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C). May be involved
CC       in a degradation pathway that converts t3LHyp to L-proline, which would
CC       allow S.novella to grow on t3LHyp as a sole carbon source.
CC       {ECO:0000269|PubMed:25608448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate + H2O;
CC         Xref=Rhea:RHEA:10320, ChEBI:CHEBI:15377, ChEBI:CHEBI:39785,
CC         ChEBI:CHEBI:57938; EC=4.2.1.77;
CC         Evidence={ECO:0000269|PubMed:25608448};
CC   -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
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DR   EMBL; CP002026; ADH87491.1; -; Genomic_DNA.
DR   RefSeq; WP_013164996.1; NC_014217.1.
DR   AlphaFoldDB; D7A0Y1; -.
DR   SMR; D7A0Y1; -.
DR   STRING; 639283.Snov_0155; -.
DR   EnsemblBacteria; ADH87491; ADH87491; Snov_0155.
DR   KEGG; sno:Snov_0155; -.
DR   eggNOG; COG3938; Bacteria.
DR   HOGENOM; CLU_036729_2_0_5; -.
DR   OMA; IMESEEY; -.
DR   OrthoDB; 559014at2; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0050346; F:trans-L-3-hydroxyproline dehydratase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR008794; Pro_racemase_fam.
DR   PANTHER; PTHR33442; PTHR33442; 1.
DR   Pfam; PF05544; Pro_racemase; 1.
DR   PIRSF; PIRSF029792; Pro_racemase; 1.
DR   SFLD; SFLDS00028; Proline_Racemase; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Lyase; Reference proteome.
FT   CHAIN           1..345
FT                   /note="Trans-3-hydroxy-L-proline dehydratase"
FT                   /id="PRO_0000433401"
FT   ACT_SITE        90
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:B9K4G4"
FT   BINDING         91..92
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B9K4G4"
FT   BINDING         252
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B9K4G4"
FT   BINDING         257..258
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B9K4G4"
SQ   SEQUENCE   345 AA;  37028 MW;  9841A83C8563BCFD CRC64;
     MRSSKVIHVV GCHAEGEVGD VIVGGVAPPP GETVWAQSRF VASDNTLRNF VLQEPRGGVF
     RHVNLLVPPK NKEAVAAWII MEPEDTPPMS GSNSICVSTV LLDTGIVPMV EPETHMVLEA
     PGGLIEATAY CKNGKAERIR VKNHPSFADK LDAKLELEGY GTLTVDTAYG GDSFCIVDAH
     ALGFSIKPDE AKDFADLGMK IVKAANQQLG FQHPTNKDWS HISFCQFAAP LTDDNGTPSG
     ANAVAIRPGK IDRSPCGTGC SARMAVLHAK GILKVGDAFV GRSIIGSRFD CRVEAETSIG
     GRPAIVPSIM GRAFITHTAQ LMVDPDDPWQ TGYRLSDTWP VWKQD