ID   T3HPD_XENTR             Reviewed;         348 AA.
AC   Q66II5;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Trans-L-3-hydroxyproline dehydratase;
DE            EC=4.2.1.77;
DE   AltName: Full=Trans-3-hydroxy-L-proline dehydratase;
GN   Name=l3hypdh;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the dehydration of trans-3-hydroxy-L-proline to
CC       delta-1-pyrroline-2-carboxylate (Pyr2C). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate + H2O;
CC         Xref=Rhea:RHEA:10320, ChEBI:CHEBI:15377, ChEBI:CHEBI:39785,
CC         ChEBI:CHEBI:57938; EC=4.2.1.77;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: In contrast to the T.cruzi proline racemase enzyme,
CC       lacks the conserved Cys at position 267 which is replaced by a Thr
CC       residue, transforming the racemase activity into dehydratase activity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
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DR   EMBL; BC081335; AAH81335.1; -; mRNA.
DR   RefSeq; NP_001008128.1; NM_001008127.1.
DR   AlphaFoldDB; Q66II5; -.
DR   SMR; Q66II5; -.
DR   STRING; 8364.ENSXETP00000037091; -.
DR   PaxDb; Q66II5; -.
DR   DNASU; 493490; -.
DR   GeneID; 493490; -.
DR   KEGG; xtr:493490; -.
DR   CTD; 112849; -.
DR   Xenbase; XB-GENE-1000732; l3hypdh.
DR   eggNOG; ENOG502QRPF; Eukaryota.
DR   InParanoid; Q66II5; -.
DR   OrthoDB; 894373at2759; -.
DR   Proteomes; UP000008143; Chromosome 8.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000017026; Expressed in 4-cell stage embryo and 12 other tissues.
DR   GO; GO:0016836; F:hydro-lyase activity; ISS:UniProtKB.
DR   GO; GO:0050346; F:trans-L-3-hydroxyproline dehydratase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR008794; Pro_racemase_fam.
DR   PANTHER; PTHR33442; PTHR33442; 1.
DR   Pfam; PF05544; Pro_racemase; 1.
DR   PIRSF; PIRSF029792; Pro_racemase; 1.
DR   SFLD; SFLDS00028; Proline_Racemase; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..348
FT                   /note="Trans-L-3-hydroxyproline dehydratase"
FT                   /id="PRO_0000288952"
FT   ACT_SITE        101
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         102..103
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         263
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         268..269
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   348 AA;  37460 MW;  D9D631AE9ECD639C CRC64;
     MAAPRLPQLP PHDGPMLSVV DMHTGGEPLR IVLSGAPAPE GRTILEKRRW VRENADWLRK
     VLMFEPRGHR DMYGALLVPG DEEEANIGVL FMHNEGYSTM CGHAIVALGR FAVDYGLVNA
     AQGPETAVNI QCPCGLIRAY VSYTGGRSGS VRFRSVPAFA FATDVTVDVP GYGKVVVDIA
     YGGAFYAFVS AETFGLDVCS SRTRDLVDVS AAVTESVKAQ VKLNHPDSDD LAFLYGTILT
     DGKDSYSEEP TANICIFAES QVDRSPTGSG VTARIALQYH KGLIQLEQIR TFKSGATGSL
     FTGKAVKETL CGNFKAVVVE VSGQAYYTGA SSFVIENKDS LKDGFLLK