T4149_TANPA
ID T4149_TANPA Reviewed; 495 AA.
AC X2ESR4;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 11-JUN-2014, sequence version 1.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=Cytochrome P450 Tp4149 {ECO:0000312|EMBL:AHM24034.1};
DE Short=Tp4149 {ECO:0000303|PubMed:24704560};
OS Tanacetum parthenium (Feverfew) (Matricaria parthenium).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Anthemidinae; Tanacetum.
OX NCBI_TaxID=127999;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=24704560; DOI=10.1016/j.ymben.2014.03.005;
RA Liu Q., Manzano D., Tanic N., Pesic M., Bankovic J., Pateraki I.,
RA Ricard L., Ferrer A., de Vos R., van de Krol S., Bouwmeester H.;
RT "Elucidation and in planta reconstitution of the parthenolide biosynthetic
RT pathway.";
RL Metab. Eng. 23C:145-153(2014).
RN [2]
RP PATHWAY, AND REVIEW.
RX PubMed=30468448; DOI=10.1039/c8np00077h;
RA Liu Y., Jing S.-X., Luo S.-H., Li S.-H.;
RT "Non-volatile natural products in plant glandular trichomes: chemistry,
RT biological activities and biosynthesis.";
RL Nat. Prod. Rep. 36:626-665(2019).
CC -!- FUNCTION: Probably involved in the biosynthesis of germacrene-derived
CC sesquiterpene lactones. {ECO:0000303|PubMed:24704560}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000303|PubMed:30468448}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: During ovary development, accumulates until the
CC stage 3 and fades out progressively to disappear at stage 6.
CC {ECO:0000269|PubMed:24704560}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; KC954156; AHM24034.1; -; mRNA.
DR AlphaFoldDB; X2ESR4; -.
DR SMR; X2ESR4; -.
DR UniPathway; UPA00213; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0051762; P:sesquiterpene biosynthetic process; IEA:UniProt.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Transmembrane; Transmembrane helix.
FT CHAIN 1..495
FT /note="Cytochrome P450 Tp4149"
FT /id="PRO_0000448401"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 437
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
FT CARBOHYD 419
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 495 AA; 55480 MW; FFABACC6D63AF595 CRC64;
MANILSLETL LISWLSSFIL MFFILKWVSF YSKTKKNLPP SPQKLPIIGN FHQLGPNPHR
SLQALSEKHG PIMLLHLGSV PMLVASNSEV AQEIMKTHDV SFASRPNSPI LNILLYGCKD
IAFAPSGEYW RQLKSIIVSN LLSSTQVKSF KNVREKEIGH MIGVIGESYG SSVDMSALLV
SLAENVICTV ALGRKFDGLK LTSLLRRYLS MFNLFSVGSY IPWLSWVDQL SGLTGRATKV
VKEFDEFLEV IIEDHANAKI NGGKDFIDML LNAQQDQTTG FAFQRDTIKA VIFDIFGGGI
DSVSTSIDWV MSELVKHPNV MKKLQKEVTE ISQGRSMVAE EDLEKMQYLK AVIKESWRLH
PPVPLLIPRK STNDVKLMGY DIQAGTQVMV NVWQIGRDPT LWDEPNEFRP ERFSKGSVNY
SGLNFEWLPF GVGRRACPGT QFGAAVIELA IANIVYKFDL ALPNGVKHED LDMSEKYGIT
VHRKNPLLVT ASPRF