T4G1E_AGEAP
ID T4G1E_AGEAP Reviewed; 37 AA.
AC P11061;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Mu-agatoxin-Aa1e {ECO:0000305};
DE Short=Mu-AGTX-Aa1e {ECO:0000305};
DE AltName: Full=Mu-agatoxin V {ECO:0000303|PubMed:2914898};
DE Short=Mu-Aga V {ECO:0000303|PubMed:2914898};
DE AltName: Full=Mu-agatoxin-5 {ECO:0000305};
OS Agelenopsis aperta (North American funnel-web spider) (Agelenopsis
OS gertschi).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Agelenidae; Agelenopsis.
OX NCBI_TaxID=6908;
RN [1]
RP PROTEIN SEQUENCE, AMIDATION AT ASN-37, FUNCTION, SUBCELLULAR LOCATION,
RP TOXIC DOSE, AND DISULFIDE BONDS.
RC TISSUE=Venom;
RX PubMed=2914898; DOI=10.1016/s0021-9258(18)94154-2;
RA Skinner W.S., Adams M.E., Quistad G.B., Kataoka H., Cesarin B.J.,
RA Enderlin F.E., Schooley D.A.;
RT "Purification and characterization of two classes of neurotoxins from the
RT funnel web spider, Agelenopsis aperta.";
RL J. Biol. Chem. 264:2150-2155(1989).
RN [2]
RP REVIEW.
RX PubMed=15066410; DOI=10.1016/j.toxicon.2004.02.004;
RA Adams M.E.;
RT "Agatoxins: ion channel specific toxins from the American funnel web
RT spider, Agelenopsis aperta.";
RL Toxicon 43:509-525(2004).
CC -!- FUNCTION: Insecticidal neurotoxin that induces an irreversible spastic
CC paralysis when injected into insects. Modifies presynaptic voltage-
CC gated sodium channels (Nav), causing them to open at the normal resting
CC potential of the nerve. This leads to spontaneous release of
CC neurotransmitter and repetitive action potentials in motor neurons.
CC {ECO:0000269|PubMed:2914898}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2914898}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:2914898}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC -!- TOXIC DOSE: LD(50) is 48 +-11 mg/kg into third stadium larvae of
CC M.sexta. {ECO:0000269|PubMed:2914898}.
CC -!- SIMILARITY: Belongs to the neurotoxin 07 (Beta/delta-agtx) family. 03
CC (aga-4) subfamily. Aga sub-subfamily. {ECO:0000305}.
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DR PIR; E32038; E32038.
DR AlphaFoldDB; P11061; -.
DR SMR; P11061; -.
DR ArachnoServer; AS000383; mu-agatoxin-Aa1e.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR016328; Beta/delta-agatoxin_fam.
DR PIRSF; PIRSF001882; Curtatoxin; 1.
DR PROSITE; PS60015; MU_AGATOXIN; 1.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Knottin; Neurotoxin; Presynaptic neurotoxin;
KW Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT PEPTIDE 1..37
FT /note="Mu-agatoxin-Aa1e"
FT /evidence="ECO:0000269|PubMed:2914898"
FT /id="PRO_0000044959"
FT MOD_RES 37
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:2914898"
FT DISULFID 2..18
FT /evidence="ECO:0000269|PubMed:2914898"
FT DISULFID 9..23
FT /evidence="ECO:0000269|PubMed:2914898"
FT DISULFID 17..33
FT /evidence="ECO:0000269|PubMed:2914898"
FT DISULFID 25..31
FT /evidence="ECO:0000269|PubMed:2914898"
SQ SEQUENCE 37 AA; 4208 MW; E742F3A18C59BBE5 CRC64;
ACVGENKQCA DWAGPHCCDG YYCTCRYFPK CICRNNN
