T4S1_HUMAN
ID T4S1_HUMAN Reviewed; 202 AA.
AC P30408; Q6IB51;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Transmembrane 4 L6 family member 1;
DE AltName: Full=Membrane component chromosome 3 surface marker 1;
DE AltName: Full=Tumor-associated antigen L6;
GN Name=TM4SF1; Synonyms=M3S1, TAAL6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1565644; DOI=10.1073/pnas.89.8.3503;
RA Marken J.S., Schieven G.L., Hellstroem I., Hellstroem K.E., Aruffo A.;
RT "Cloning and expression of the tumor-associated antigen L6.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:3503-3507(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TOPOLOGY.
RX PubMed=7510285; DOI=10.1016/s0021-9258(17)37298-8;
RA Marken J.S., Bajorath J., Edwards C.P., Farr A.G., Schieven G.L.,
RA Hellstroem I., Hellstroem K.E., Aruffo A.;
RT "Membrane topology of the L6 antigen and identification of the protein
RT epitope recognized by the L6 monoclonal antibody.";
RL J. Biol. Chem. 269:7397-7401(1994).
RN [5]
RP MUTAGENESIS OF CYS-202, INTERACTION WITH SDCBP2, AND SUBCELLULAR LOCATION.
RX PubMed=11102519; DOI=10.1091/mbc.11.12.4217;
RA Borrell-Pages M., Fernandez-Larrea J., Borroto A., Rojo F., Baselga J.,
RA Arribas J.;
RT "The carboxy-terminal cysteine of the tetraspanin L6 antigen is required
RT for its interaction with SITAC, a novel PDZ protein.";
RL Mol. Biol. Cell 11:4217-4225(2000).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBUNIT: Present in high molecular weight complexes in tumor cells.
CC Interacts with SDCBP2 (PubMed:11102519). {ECO:0000269|PubMed:11102519}.
CC -!- INTERACTION:
CC P30408; Q9H190: SDCBP2; NbExp=3; IntAct=EBI-714256, EBI-742426;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC Note=Colocalizes with SDCBP2 in the apical region of the cell
CC (PubMed:11102519). {ECO:0000269|PubMed:11102519}.
CC -!- TISSUE SPECIFICITY: Highly expressed in lung, breast, colon and ovarian
CC carcinomas. It is also present on some normal cells, endothelial cells
CC in particular.
CC -!- SIMILARITY: Belongs to the L6 tetraspanin family. {ECO:0000305}.
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DR EMBL; M90657; AAA36158.1; -; mRNA.
DR EMBL; CR456953; CAG33234.1; -; mRNA.
DR EMBL; BC008442; AAH08442.1; -; mRNA.
DR EMBL; BC010166; AAH10166.1; -; mRNA.
DR CCDS; CCDS3143.1; -.
DR PIR; A42926; A42926.
DR RefSeq; NP_055035.1; NM_014220.2.
DR AlphaFoldDB; P30408; -.
DR BioGRID; 110249; 9.
DR IntAct; P30408; 8.
DR MINT; P30408; -.
DR STRING; 9606.ENSP00000304277; -.
DR GlyGen; P30408; 2 sites.
DR PhosphoSitePlus; P30408; -.
DR SwissPalm; P30408; -.
DR BioMuta; TM4SF1; -.
DR DMDM; 267068; -.
DR jPOST; P30408; -.
DR MassIVE; P30408; -.
DR MaxQB; P30408; -.
DR PaxDb; P30408; -.
DR PeptideAtlas; P30408; -.
DR PRIDE; P30408; -.
DR ProteomicsDB; 54663; -.
DR ABCD; P30408; 1 sequenced antibody.
DR Antibodypedia; 1003; 280 antibodies from 32 providers.
DR DNASU; 4071; -.
DR Ensembl; ENST00000305366.8; ENSP00000304277.3; ENSG00000169908.12.
DR GeneID; 4071; -.
DR KEGG; hsa:4071; -.
DR MANE-Select; ENST00000305366.8; ENSP00000304277.3; NM_014220.3; NP_055035.1.
DR UCSC; uc003exb.2; human.
DR CTD; 4071; -.
DR DisGeNET; 4071; -.
DR GeneCards; TM4SF1; -.
DR HGNC; HGNC:11853; TM4SF1.
DR HPA; ENSG00000169908; Low tissue specificity.
DR MIM; 191155; gene.
DR neXtProt; NX_P30408; -.
DR OpenTargets; ENSG00000169908; -.
DR PharmGKB; PA36554; -.
DR VEuPathDB; HostDB:ENSG00000169908; -.
DR eggNOG; ENOG502RY7H; Eukaryota.
DR GeneTree; ENSGT01030000234590; -.
DR HOGENOM; CLU_087168_1_0_1; -.
DR InParanoid; P30408; -.
DR OMA; ICRPCCY; -.
DR OrthoDB; 922490at2759; -.
DR PhylomeDB; P30408; -.
DR TreeFam; TF331371; -.
DR PathwayCommons; P30408; -.
DR SignaLink; P30408; -.
DR BioGRID-ORCS; 4071; 17 hits in 1075 CRISPR screens.
DR ChiTaRS; TM4SF1; human.
DR GeneWiki; TM4SF1; -.
DR GenomeRNAi; 4071; -.
DR Pharos; P30408; Tbio.
DR PRO; PR:P30408; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; P30408; protein.
DR Bgee; ENSG00000169908; Expressed in vena cava and 202 other tissues.
DR ExpressionAtlas; P30408; baseline and differential.
DR Genevisible; P30408; HS.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0001825; P:blastocyst formation; IEA:Ensembl.
DR InterPro; IPR008661; L6_membrane.
DR PANTHER; PTHR14198; PTHR14198; 1.
DR Pfam; PF05805; L6_membrane; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..202
FT /note="Transmembrane 4 L6 family member 1"
FT /id="PRO_0000219295"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:7510285"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 31..49
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:7510285"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 71..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:7510285"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 115..161
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:7510285"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 183..202
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:7510285"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 159
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 202
FT /note="C->G: Abolishes interaction with SDCBP2. Loss of
FT colocalization with SDCBP2 at the apical region of the
FT cell."
FT /evidence="ECO:0000269|PubMed:11102519"
SQ SEQUENCE 202 AA; 21632 MW; 80D82C47902B8CD4 CRC64;
MCYGKCARCI GHSLVGLALL CIAANILLYF PNGETKYASE NHLSRFVWFF SGIVGGGLLM
LLPAFVFIGL EQDDCCGCCG HENCGKRCAM LSSVLAALIG IAGSGYCVIV AALGLAEGPL
CLDSLGQWNY TFASTEGQYL LDTSTWSECT EPKHIVEWNV SLFSILLALG GIEFILCLIQ
VINGVLGGIC GFCCSHQQQY DC
