T4S4_HUMAN
ID T4S4_HUMAN Reviewed; 202 AA.
AC P48230; B2RDA4;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transmembrane 4 L6 family member 4;
DE AltName: Full=Intestine and liver tetraspan membrane protein;
DE Short=IL-TMP;
GN Name=TM4SF4; Synonyms=ILTMP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Intestine, and Liver;
RX PubMed=7665614; DOI=10.1074/jbc.270.37.21907;
RA Wice B.M., Gordon J.I.;
RT "A tetraspan membrane glycoprotein produced in the human intestinal
RT epithelium and liver that can regulate cell density-dependent
RT proliferation.";
RL J. Biol. Chem. 270:21907-21918(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
CC -!- FUNCTION: Regulates the adhesive and proliferative status of intestinal
CC epithelial cells. Can mediate density-dependent cell proliferation.
CC -!- INTERACTION:
CC P48230; Q8WW43: APH1B; NbExp=3; IntAct=EBI-8650934, EBI-2606497;
CC P48230; P11912: CD79A; NbExp=3; IntAct=EBI-8650934, EBI-7797864;
CC P48230; Q6ZS10: CLEC17A; NbExp=3; IntAct=EBI-8650934, EBI-11977093;
CC P48230; Q8TAZ6: CMTM2; NbExp=3; IntAct=EBI-8650934, EBI-2339374;
CC P48230; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-8650934, EBI-781551;
CC P48230; Q969F0: FATE1; NbExp=3; IntAct=EBI-8650934, EBI-743099;
CC P48230; O15552: FFAR2; NbExp=3; IntAct=EBI-8650934, EBI-2833872;
CC P48230; P08034: GJB1; NbExp=3; IntAct=EBI-8650934, EBI-17565645;
CC P48230; Q9NTQ9: GJB4; NbExp=3; IntAct=EBI-8650934, EBI-12831526;
CC P48230; O95377: GJB5; NbExp=3; IntAct=EBI-8650934, EBI-3909454;
CC P48230; Q96P66: GPR101; NbExp=3; IntAct=EBI-8650934, EBI-17935713;
CC P48230; O60883: GPR37L1; NbExp=3; IntAct=EBI-8650934, EBI-2927498;
CC P48230; O15529: GPR42; NbExp=3; IntAct=EBI-8650934, EBI-18076404;
CC P48230; Q8TED1: GPX8; NbExp=3; IntAct=EBI-8650934, EBI-11721746;
CC P48230; O95279: KCNK5; NbExp=3; IntAct=EBI-8650934, EBI-3934936;
CC P48230; Q8TAF8: LHFPL5; NbExp=3; IntAct=EBI-8650934, EBI-2820517;
CC P48230; P35372-10: OPRM1; NbExp=3; IntAct=EBI-8650934, EBI-12807478;
CC P48230; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-8650934, EBI-18159983;
CC P48230; Q0VAB0: TBXA2R; NbExp=3; IntAct=EBI-8650934, EBI-18271435;
CC P48230; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-8650934, EBI-8638294;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Jejunum and liver.
CC -!- PTM: N-glycosylated. Glycosylation is required for the growth
CC inhibitory effect. {ECO:0000269|PubMed:19159218}.
CC -!- SIMILARITY: Belongs to the L6 tetraspanin family. {ECO:0000305}.
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DR EMBL; U31449; AAA74745.1; -; mRNA.
DR EMBL; BT007430; AAP36098.1; -; mRNA.
DR EMBL; AK315464; BAG37851.1; -; mRNA.
DR EMBL; CH471052; EAW78871.1; -; Genomic_DNA.
DR EMBL; BC001386; AAH01386.1; -; mRNA.
DR CCDS; CCDS46932.1; -.
DR RefSeq; NP_004608.1; NM_004617.3.
DR AlphaFoldDB; P48230; -.
DR BioGRID; 112959; 23.
DR IntAct; P48230; 22.
DR MINT; P48230; -.
DR STRING; 9606.ENSP00000305852; -.
DR TCDB; 8.A.75.1.1; the transmembrane 4 l6 (tm4l6) family.
DR GlyGen; P48230; 2 sites.
DR iPTMnet; P48230; -.
DR PhosphoSitePlus; P48230; -.
DR BioMuta; TM4SF4; -.
DR DMDM; 1352457; -.
DR MassIVE; P48230; -.
DR PaxDb; P48230; -.
DR PeptideAtlas; P48230; -.
DR PRIDE; P48230; -.
DR ProteomicsDB; 55873; -.
DR Antibodypedia; 33572; 138 antibodies from 21 providers.
DR DNASU; 7104; -.
DR Ensembl; ENST00000305354.5; ENSP00000305852.4; ENSG00000169903.7.
DR GeneID; 7104; -.
DR KEGG; hsa:7104; -.
DR MANE-Select; ENST00000305354.5; ENSP00000305852.4; NM_004617.4; NP_004608.1.
DR UCSC; uc003exd.3; human.
DR CTD; 7104; -.
DR DisGeNET; 7104; -.
DR GeneCards; TM4SF4; -.
DR HGNC; HGNC:11856; TM4SF4.
DR HPA; ENSG00000169903; Group enriched (gallbladder, intestine, liver).
DR MIM; 606567; gene.
DR neXtProt; NX_P48230; -.
DR OpenTargets; ENSG00000169903; -.
DR PharmGKB; PA36557; -.
DR VEuPathDB; HostDB:ENSG00000169903; -.
DR eggNOG; ENOG502QVGK; Eukaryota.
DR GeneTree; ENSGT01030000234590; -.
DR HOGENOM; CLU_087168_1_0_1; -.
DR InParanoid; P48230; -.
DR OMA; CGCCGNQ; -.
DR OrthoDB; 922490at2759; -.
DR PhylomeDB; P48230; -.
DR TreeFam; TF331371; -.
DR PathwayCommons; P48230; -.
DR SignaLink; P48230; -.
DR BioGRID-ORCS; 7104; 11 hits in 1061 CRISPR screens.
DR ChiTaRS; TM4SF4; human.
DR GenomeRNAi; 7104; -.
DR Pharos; P48230; Tbio.
DR PRO; PR:P48230; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; P48230; protein.
DR Bgee; ENSG00000169903; Expressed in gall bladder and 99 other tissues.
DR ExpressionAtlas; P48230; baseline and differential.
DR Genevisible; P48230; HS.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0042246; P:tissue regeneration; IEA:Ensembl.
DR InterPro; IPR008661; L6_membrane.
DR PANTHER; PTHR14198; PTHR14198; 1.
DR Pfam; PF05805; L6_membrane; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..202
FT /note="Transmembrane 4 L6 family member 4"
FT /id="PRO_0000219299"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..45
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..158
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..202
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 202 AA; 21396 MW; 402742A2F5F8598F CRC64;
MCTGGCARCL GGTLIPLAFF GFLANILLFF PGGKVIDDND HLSQEIWFFG GILGSGVLMI
FPALVFLGLK NNDCCGCCGN EGCGKRFAMF TSTIFAVVGF LGAGYSFIIS AISINKGPKC
LMANSTWGYP FHDGDYLNDE ALWNKCREPL NVVPWNLTLF SILLVVGGIQ MVLCAIQVVN
GLLGTLCGDC QCCGCCGGDG PV
