ID   T5AT_TAXCU              Reviewed;         439 AA.
AC   Q9M6F0;
DT   12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Taxadien-5-alpha-ol O-acetyltransferase;
DE            EC=2.3.1.162;
DE   AltName: Full=Taxa-4(20),11(12)-dien-5alpha-ol-O-acetyltransferase;
DE            Short=Taxadienol acetyltransferase;
GN   Name=TAT;
OS   Taxus cuspidata (Japanese yew).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Taxaceae;
OC   Taxus.
OX   NCBI_TaxID=99806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
RX   PubMed=10666320; DOI=10.1006/abbi.1999.1609;
RA   Walker K., Schoendorf A., Croteau R.B.;
RT   "Molecular cloning of a taxa-4(20),11(12)-dien-5alpha-ol-O-acetyl
RT   transferase cDNA from Taxus and functional expression in Escherichia
RT   coli.";
RL   Arch. Biochem. Biophys. 374:371-380(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-
CC         4(20),11-dien-5alpha-yl acetate; Xref=Rhea:RHEA:22028,
CC         ChEBI:CHEBI:30038, ChEBI:CHEBI:30042, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.1.162;
CC   -!- PATHWAY: Alkaloid biosynthesis; taxol biosynthesis; 10-deacetyl-2-
CC       debenzoylbaccatin III from taxa-4(20),11-dien-5alpha-ol: step 1/3.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF190130; AAF34254.1; -; mRNA.
DR   PIR; T52321; T52321.
DR   AlphaFoldDB; Q9M6F0; -.
DR   SMR; Q9M6F0; -.
DR   KEGG; ag:AAF34254; -.
DR   BRENDA; 2.3.1.162; 6225.
DR   UniPathway; UPA00842; UER00808.
DR   GO; GO:0050638; F:taxadien-5-alpha-ol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042617; P:paclitaxel biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Direct protein sequencing; Taxol biosynthesis;
KW   Transferase.
FT   CHAIN           1..439
FT                   /note="Taxadien-5-alpha-ol O-acetyltransferase"
FT                   /id="PRO_0000147358"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        373
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   439 AA;  49080 MW;  7095CAF119BB8DFF CRC64;
     MEKTDLHVNL IEKVMVGPSP PLPKTTLQLS SIDNLPGVRG SIFNALLIYN ASPSPTMISA
     DPAKPIREAL AKILVYYPPF AGRLRETENG DLEVECTGEG AMFLEAMADN ELSVLGDFDD
     SNPSFQQLLF SLPLDTNFKD LSLLVVQVTR FTCGGFVVGV SFHHGVCDGR GAAQFLKGLA
     EMARGEVKLS LEPIWNRELV KLDDPKYLQF FHFEFLRAPS IVEKIVQTYF IIDFETINYI
     KQSVMEECKE FCSSFEVASA MTWIARTRAF QIPESEYVKI LFGMDMRNSF NPPLPSGYYG
     NSIGTACAVD NVQDLLSGSL LRAIMIIKKS KVSLNDNFKS RAVVKPSELD VNMNHENVVA
     FADWSRLGFD EVDFGWGNAV SVSPVQQQSA LAMQNYFLFL KPSKNKPDGI KILMFLPLSK
     MKSFKIEMEA MMKKYVAKV