ID   T5AT_TAXWC              Reviewed;         439 AA.
AC   Q8S9G6;
DT   12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Taxadien-5-alpha-ol O-acetyltransferase;
DE            EC=2.3.1.162;
DE   AltName: Full=Taxa-4(20),11(12)-dien-5alpha-ol-O-acetyltransferase;
DE            Short=Taxadienol acetyltransferase;
OS   Taxus wallichiana var. chinensis (Chinese yew) (Taxus chinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Taxaceae;
OC   Taxus.
OX   NCBI_TaxID=29808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Wang W., Song S., Cheng K.;
RT   "Cloning of a taxa-4(20),11(12)-dien-5alpha-ol-O-acetyltransferase cDNA
RT   from Taxus chinensis and functional expression in Escherichia coli.";
RL   Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-
CC         4(20),11-dien-5alpha-yl acetate; Xref=Rhea:RHEA:22028,
CC         ChEBI:CHEBI:30038, ChEBI:CHEBI:30042, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.1.162;
CC   -!- PATHWAY: Alkaloid biosynthesis; taxol biosynthesis; 10-deacetyl-2-
CC       debenzoylbaccatin III from taxa-4(20),11-dien-5alpha-ol: step 1/3.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AY078285; AAL78754.1; -; mRNA.
DR   AlphaFoldDB; Q8S9G6; -.
DR   SMR; Q8S9G6; -.
DR   BioCyc; MetaCyc:MON-13400; -.
DR   UniPathway; UPA00842; UER00808.
DR   GO; GO:0050638; F:taxadien-5-alpha-ol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042617; P:paclitaxel biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Taxol biosynthesis; Transferase.
FT   CHAIN           1..439
FT                   /note="Taxadien-5-alpha-ol O-acetyltransferase"
FT                   /id="PRO_0000147357"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        373
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   439 AA;  49183 MW;  59EC6E807989C843 CRC64;
     MEKTDLHVNL NEKVMVGPSL PLPKTTLQLS SIDNLPGVRG SIFNALLIYN ASPSPTMVSA
     DPAKLIREAL AKILVYYPPF AGRLRETENG DLEVECTGEG AMFLEAMADN ELSVLGDFDD
     SNPSFQQLLF SLPLDTNFKD LPLLVVQVTR FTCGGFVVGV SFHHGVCDGR GAAQFLKGLA
     EMARGEVKLS LEPIWNRELV KLDDPKYLQF FHFEFLRAPS IVEKIVQTYF IIDFETINYI
     KQSVMEECKE FCSSFEVASA MTWIARTRAF QIPESEYVKI LFGMDMRNSF NPPLPSGYYG
     NSIGTACAVD NVQDLLSGSL LRAIMIIKKS KVSLNDNFKS RAVVKPSELD VNMNHENVVA
     FADWSRLGFD EVDFGWGNAV SVSPVQQQCE LAMQNYFLFL KPSKNKPDGI KILMFLPLSK
     MKSFKIEMEA MMKKYVAKV