T7L1A_DANRE
ID T7L1A_DANRE Reviewed; 560 AA.
AC Q9YHE8;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Transcription factor 7-like 1-A;
DE AltName: Full=HMG box transcription factor 3-A;
DE Short=TCF-3;
DE Short=TCF-3-A;
DE Short=zTcf-3;
DE AltName: Full=Protein headless;
GN Name=tcf7l1a; Synonyms=hdl, tcf3, tcf3a;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=9784608; DOI=10.1016/s0925-4773(98)00132-4;
RA Pelegri F., Maischein H.-M.;
RT "Function of zebrafish beta-catenin and TCF-3 in dorsoventral patterning.";
RL Mech. Dev. 77:63-74(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11057671; DOI=10.1038/35038097;
RA Kim C.-H., Oda T., Itoh M., Jiang D., Artinger K.B.,
RA Chandrasekharappa S.C., Driever W., Chitnis A.B.;
RT "Repressor activity of Headless/Tcf3 is essential for vertebrate head
RT formation.";
RL Nature 407:913-916(2000).
RN [4]
RP FUNCTION.
RX PubMed=11784107; DOI=10.1006/dbio.2001.0515;
RA Dorsky R.I., Sheldahl L.C., Moon R.T.;
RT "A transgenic Lef1/beta-catenin-dependent reporter is expressed in
RT spatially restricted domains throughout zebrafish development.";
RL Dev. Biol. 241:229-237(2002).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12642497; DOI=10.1242/dev.00402;
RA Dorsky R.I., Itoh M., Moon R.T., Chitnis A.;
RT "Two tcf3 genes cooperate to pattern the zebrafish brain.";
RL Development 130:1937-1947(2003).
RN [6]
RP FUNCTION.
RX PubMed=15151990; DOI=10.1242/dev.01171;
RA Thorpe C.J., Moon R.T.;
RT "nemo-like kinase is an essential co-activator of Wnt signaling during
RT early zebrafish development.";
RL Development 131:2899-2909(2004).
RN [7]
RP INTERACTION WITH TLE3A.
RX PubMed=25371059; DOI=10.1038/ncomms6368;
RA Lu F.I., Sun Y.H., Wei C.Y., Thisse C., Thisse B.;
RT "Tissue-specific derepression of TCF/LEF controls the activity of the
RT Wnt/beta-catenin pathway.";
RL Nat. Commun. 5:5368-5368(2014).
CC -!- FUNCTION: Participates in the Wnt signaling pathway. Probably binds to
CC DNA and acts as a repressor in the absence of ctnnb1, and possibly as
CC an activator in its presence. Required early in development for dorsal
CC specific gene activation and during gastrulation for repression of
CC dorsal-specific genes in the marginal zone. Required for head formation
CC and forebrain specification. Regulates anterior-posterior patterning in
CC the neuroectoderm by repressing posterior neural fates.
CC {ECO:0000269|PubMed:11057671, ECO:0000269|PubMed:11784107,
CC ECO:0000269|PubMed:12642497, ECO:0000269|PubMed:15151990,
CC ECO:0000269|PubMed:9784608}.
CC -!- SUBUNIT: Interacts with tle3a/gro2. {ECO:0000269|PubMed:25371059}.
CC -!- INTERACTION:
CC Q9YHE8; Q66K89: E4F1; Xeno; NbExp=2; IntAct=EBI-6979298, EBI-1227043;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:11057671}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and throughout embryogenesis.
CC Expressed in the dorsal mesoderm at 6 hours post fertilization (6 hpf),
CC and this expression is maintained in the axial mesoderm during
CC gastrulation and early somitogenesis. Strongly expressed in the
CC prospective neuroectoderm at 8 hpf. Expression persists in the
CC neuroectoderm and increases in the tail bud at the end of gastrulation,
CC at 10 hpf. Enriched in the prechordal mesoderm and developing notochord
CC and in ventromedial regions of the brain and neural tube during the
CC early somite stages. Expression remains elevated within the tail bud,
CC forebrain, the midbrain-hindbrain boundary and in the developing otic
CC vesicles as somitogenesis progresses. {ECO:0000269|PubMed:12642497,
CC ECO:0000269|PubMed:9784608}.
CC -!- INDUCTION: By ctnnb1. {ECO:0000269|PubMed:9784608}.
CC -!- SIMILARITY: Belongs to the TCF/LEF family. {ECO:0000305}.
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DR EMBL; AF097410; AAC64609.1; -; mRNA.
DR EMBL; BC053135; AAH53135.1; -; mRNA.
DR RefSeq; NP_571344.1; NM_131269.2.
DR AlphaFoldDB; Q9YHE8; -.
DR SMR; Q9YHE8; -.
DR BioGRID; 78699; 6.
DR IntAct; Q9YHE8; 4.
DR MINT; Q9YHE8; -.
DR STRING; 7955.ENSDARP00000090035; -.
DR PaxDb; Q9YHE8; -.
DR Ensembl; ENSDART00000099262; ENSDARP00000090035; ENSDARG00000038159.
DR GeneID; 30523; -.
DR KEGG; dre:30523; -.
DR CTD; 30523; -.
DR ZFIN; ZDB-GENE-980605-30; tcf7l1a.
DR eggNOG; KOG3248; Eukaryota.
DR GeneTree; ENSGT00940000157038; -.
DR HOGENOM; CLU_013229_4_0_1; -.
DR InParanoid; Q9YHE8; -.
DR OMA; HPLSWLV; -.
DR OrthoDB; 807716at2759; -.
DR PhylomeDB; Q9YHE8; -.
DR TreeFam; TF318448; -.
DR Reactome; R-DRE-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-DRE-3769402; Deactivation of the beta-catenin transactivating complex.
DR Reactome; R-DRE-4086398; Ca2+ pathway.
DR Reactome; R-DRE-4641265; Repression of WNT target genes.
DR Reactome; R-DRE-8853884; Transcriptional Regulation by VENTX.
DR Reactome; R-DRE-8951430; RUNX3 regulates WNT signaling.
DR SignaLink; Q9YHE8; -.
DR PRO; PR:Q9YHE8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 10.
DR Bgee; ENSDARG00000038159; Expressed in early embryo and 49 other tissues.
DR ExpressionAtlas; Q9YHE8; baseline and differential.
DR GO; GO:1990907; C:beta-catenin-TCF complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:ZFIN.
DR GO; GO:0008013; F:beta-catenin binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IDA:ZFIN.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0010456; P:cell proliferation in dorsal spinal cord; IGI:ZFIN.
DR GO; GO:0060900; P:embryonic camera-type eye formation; IMP:ZFIN.
DR GO; GO:0048596; P:embryonic camera-type eye morphogenesis; IGI:ZFIN.
DR GO; GO:0060847; P:endothelial cell fate specification; IMP:ZFIN.
DR GO; GO:0030900; P:forebrain development; IGI:ZFIN.
DR GO; GO:0048699; P:generation of neurons; IGI:ZFIN.
DR GO; GO:0060323; P:head morphogenesis; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IMP:ZFIN.
DR GO; GO:0030901; P:midbrain development; IMP:ZFIN.
DR GO; GO:0060829; P:negative regulation of canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation; IGI:ZFIN.
DR GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IMP:ZFIN.
DR GO; GO:2000054; P:negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification; IGI:ZFIN.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IGI:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IGI:ZFIN.
DR GO; GO:0021903; P:rostrocaudal neural tube patterning; IMP:ZFIN.
DR Gene3D; 1.10.30.10; -; 1.
DR Gene3D; 4.10.900.10; -; 1.
DR InterPro; IPR027397; Catenin-bd_sf.
DR InterPro; IPR013558; CTNNB1-bd_N.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR024940; TCF/LEF.
DR InterPro; IPR028778; Tcf7l1.
DR PANTHER; PTHR10373; PTHR10373; 1.
DR PANTHER; PTHR10373:SF25; PTHR10373:SF25; 1.
DR Pfam; PF08347; CTNNB1_binding; 1.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..560
FT /note="Transcription factor 7-like 1-A"
FT /id="PRO_0000048621"
FT DNA_BIND 329..397
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 395..513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..47
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..77
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 301..315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..453
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 560 AA; 61459 MW; DC35C50EA4D61232 CRC64;
MPQLNGGGGD DLGANDELIS FKDEGEQEEK ISENVSSERD LDEVKSSLVN ESENNSSSSD
SEQTDRRPRP RPDLESYEKQ REYFAEALRR QQDGGFFKGP PYAGYPFLMI PDITNPYLSN
GSLSPSTRTY LQMKWPLLDV PASAALKDSR SPTPGHLSNK VPVVQHPHHV HPLTPLITYS
NEHFSPGTPP SHLSPEILDP KTGIPRTPHP SELSPYYPLS PGAVGQIPHP LGWLVPQQGQ
HMYSIPPGGF RHPYPALAMN ASMSSLVSSR FSPHMVPHPP HGLHQTGIPH PAIVSPAIKQ
EPNGESPSNS THGKPSVPVK KEEEKKPHIK KPLNAFMLYM KEMRAKVVAE CTLKESAAIN
QILGRRWHSL SREEQAKYYE LARKERQLHS QLYPGWSARD NYGKRKKRKR DCKSDSPSES
NFSPQPKKQC VPYLSSEKMC DSPTSSHGSM LDSPATPSAA LASPAAPAAT HSEQAQPLSL
TTKPEGRAHH NHPHFPLPGK SSGSGSGSSM ALHSLSRPIP FTSLPPSLLG PNSPFHQAAL
HSHHALLQTQ PLSLVTKSVE
